The integration of multi-‘omic datasets into complex systems-wide assessments has become a mainstay in immunologic investigation. This focus on high-dimensional data collection and analysis was on full display in the investigation of COVID-19, the respiratory illness resulting from infection by the novel coronavirus SARS-CoV-2. Particularly in the area of B cell biology, tremendous efforts in both cellular and serologic investigation have resulted in an increasingly detailed mapping of the coordinated effector, memory, and antibody secreting cell responses that underpin the development of humoral immunity in response to primary viral infection. Further, the rapid development and deployment of effective vaccines has allowed for the assessment of developing memory responses across a wide variety of immune contexts, including in patients with compromised immune function. The result has been a period of rapid gains in the understanding of B cell biology unrestricted to the study of COVID-19. Here, we outline the systems-level technologies that have been routinely implemented in these investigations throughout the pandemic, and discuss how their use has led to clear and applicable gains in pursuance of the amelioration of human infectious disease and beyond.

中文翻译：

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COVID-19 中 B 细胞的系统生物学

将多组学数据集整合到复杂的全系统评估中已成为免疫学研究的支柱。这种对高维数据收集和分析的重视在对新型冠状病毒 SARS-CoV-2 感染引起的呼吸道疾病 COVID-19 的调查中得到了充分体现。特别是在 B 细胞生物学领域，细胞和血清学研究方面的巨大努力已经导致对协调效应器、记忆和抗体分泌细胞反应的绘制越来越详细，这些细胞反应支撑着针对原发性病毒感染的体液免疫的发展。此外，有效疫苗的快速开发和部署使得可以评估各种免疫环境中的记忆反应，包括免疫功能受损的患者。其结果是，人们对 B 细胞生物学的理解在一段时期内迅速取得进展，而不仅限于 COVID-19 的研究。在这里，我们概述了在整个大流行期间这些调查中常规实施的系统级技术，并讨论了它们的使用如何在改善人类传染病及其他方面带来了明确和适用的成果。