Patients diagnosed with T1a cancer undergo partial nephrectomy to remove the tumors. In the process of removing the tumors, loss of kidney volume is inevitable, and current surgical methods focus solely on hemostasis and wound closure. Here, we developed an implantable form of decellularized extracellular matrix sponge to target both hemostasis and wound healing at the lesion site. A porous form of kidney decellularized matrix was achieved by fabricating a chemically cross-linked cryogel followed by lyophilization. The prepared kidney decellularized extracellular matrix sponge (kdES) was then characterized for features relevant to a hemostasis as well as a biocompatible and degradable biomaterial. Finally, histological evaluations were made after implantation in rat kidney incision model. Both gelatin sponge and kdES displayed excellent hemocompatibility and biocompatibility. However, after a 4-week observation period, kdES exhibited more favorable wound healing results at the lesion site. This suggests a promising potential for kdES as a supportive material in facilitating wound closure during partial nephrectomy surgery. KdES not only achieved rapid hemostasis for managing renal hemorrhage that is comparable to commercial hemostatic sponges, but also demonstrated superior wound healing outcomes.

中文翻译：

---

降解控制的组织细胞外海绵用于肾损伤后快速止血和伤口修复

诊断患有 T1a 癌症的患者接受部分肾切除术以切除肿瘤。在切除肿瘤的过程中，肾脏体积的损失是不可避免的，目前的手术方法仅集中于止血和伤口闭合。在这里，我们开发了一种可植入形式的脱细胞细胞外基质海绵，以实现病变部位的止血和伤口愈合。通过制造化学交联的冷冻凝胶并随后冻干，获得了多孔形式的肾脏脱细胞基质。然后对制备的肾脏脱细胞细胞外基质海绵（kdES）进行了与止血相关的特征以及生物相容性和可降解生物材料的表征。最后，植入大鼠肾切口模型后进行组织学评价。明胶海绵和kdES均表现出优异的血液相容性和生物相容性。然而，经过 4 周的观察期后，kdES 在病变部位表现出更有利的伤口愈合结果。这表明 kdES 作为支持材料在肾部分切除手术期间促进伤口闭合方面具有广阔的前景。 KdES 不仅能够实现与商业止血海绵相当的快速止血来治疗肾出血，而且还表现出优异的伤口愈合效果。