With the emergence of microRNA (miRNA) as a promising biomarker in cancer diagnosis, it is significant to develop multiple analyses of miRNAs. However, it still faces difficulties in ensuring the sensitivity and accuracy during multiplex detection owing to the low abundance and experimental deviation of miRNAs. In this work, a flexible-arranged biomimetic array integrated with parallel entropy-driven circuits (EDCs) was developed for ultrasensitive, multiplex, reliable, and high-throughput detection of miRNAs. The biomimetic array was fabricated by arrangement of various photonic crystals (PCs) for adjustable photonic band gaps (PBGs) and specific fluorescence enhancement. Meanwhile, two cancer-related miRNAs and one reference miRNA were introduced as multiple analytes as a proof-of-concept. The parallel EDCs with negligible crosstalk were designed based on the modular property. Because of the one-to-one match between the emitted fluorescence of parallel EDCs and the PBGs of the flexible-arranged biomimetic array, the generated fluorescence signal triggered by target miRNAs can be enhanced on the corresponding domain of the array. Furthermore, the amplified signal of the array was detected with high-throughput scanning, which could reveal specific information on cancer-related miRNAs as well as reference miRNA, enhancing the abundance and reliability of the analysis. The proposed array has the merits of a modular design, flexible deployment, simple operation (nonenzymatic and isothermal), improved accuracy, high sensitivity, and multiplex analysis, showing potential in disease diagnosis.

中文翻译：

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灵活排列的仿生阵列与并行熵驱动电路集成，可实现癌症相关 MicroRNA 的超灵敏、多重且可靠的检测

随着 microRNA (miRNA) 作为一种有前途的癌症诊断生物标志物的出现，开发 miRNA 的多重分析具有重要意义。然而，由于miRNA丰度低且存在实验偏差，多重检测的灵敏度和准确性仍面临困难。在这项工作中，开发了一种与并行熵驱动电路（EDC）集成的灵活排列的仿生阵列，用于超灵敏、多重、可靠和高通量的 miRNA 检测。该仿生阵列是通过排列各种光子晶体（PC）来制造的，以实现可调节的光子带隙（PBG）和特定的荧光增强。同时，引入了两种与癌症相关的 miRNA 和一种参考 miRNA 作为多种分析物作为概念验证。基于模块化特性设计了具有可忽略串扰的并行EDC。由于平行EDC发射的荧光与灵活排列的仿生阵列的PBG之间一对一匹配，因此可以在阵列的相应域上增强由目标miRNA触发的产生的荧光信号。此外，通过高通量扫描检测阵列的放大信号，可以揭示癌症相关miRNA以及参考miRNA的特定信息，从而提高分析的丰度和可靠性。所提出的阵列具有模块化设计、灵活部署、操作简单（非酶促和等温）、提高的准确性、高灵敏度和多重分析的优点，在疾病诊断中显示出潜力。