The quantitative exploration of cellular osmotic responses and a thorough analysis of osmotic pressure-responsive cellular behaviors are poised to offer novel clinical insights into current research. This underscores a paradigm shift in the long-standing approach of colorimetric measurements triggered by red cell lysis. In this study, we engineered a purpose-driven optofluidic platform to facilitate the goal. Specifically, creating photocurable hydrogel traps surmounts a persistent challenge─optical signal interference from fluid disturbances. This achievement ensures a stable spatial phase of cells and the acquisition of optical signals for accurate osmotic response analysis at the single-cell level. Leveraging a multigradient microfluidic system, we constructed gradient osmotic hydrogel traps and developed an imaging recognition algorithm, empowering comprehensive analysis of cellular behaviors. Notably, this system has successfully and precisely analyzed individual and clustered cellular responses within the osmotic dimension. Prospective clinical testing has further substantiated its feasibility and performance in that it demonstrates an accuracy of 92% in discriminating complete hemolysis values (n = 25) and 100% in identifying initial hemolysis values (n = 25). Foreseeably, this strategy should promise to advance osmotic pressure-related cellular response analysis, benefiting further investigation and diagnosis of related blood diseases, blood quality, drug development, etc.

中文翻译：

---

用于定量血细胞渗透分析的梯度水凝胶空间捕获光学细胞分析

细胞渗透反应的定量探索和渗透压响应细胞行为的彻底分析将为当前研究提供新的临床见解。这强调了由红细胞裂解引发的长期比色测量方法的范式转变。在这项研究中，我们设计了一个目的驱动的光流控平台来实现这一目标。具体来说，创建光固化水凝胶陷阱克服了一个持续存在的挑战——流体扰动造成的光信号干扰。这一成就确保了细胞稳定的空间相位和光信号的采集，以在单细胞水平上进行准确的渗透响应分析。利用多梯度微流体系统，我们构建了梯度渗透水凝胶陷阱并开发了成像识别算法，从而能够对细胞行为进行全面分析。值得注意的是，该系统成功且精确地分析了渗透维度内的个体和集群细胞反应。前瞻性临床测试进一步证实了其可行性和性能，它在区分完全溶血值 ( n = 25)方面的准确度为 92%，在识别初始溶血值 ( n = 25) 方面的准确度为 100%。可以预见，这一策略有望推进渗透压相关的细胞反应分析，有利于相关血液疾病、血液质量、药物开发等的进一步研究和诊断。