Phosphorus, essential for growth/productivity of plants, is acquired through roots in the form of inorganic phosphate (Pi). The molecular mechanism of low Pi tolerance in plants is still not fully understood. Functions of QTL in Pi-deficiency tolerance in rice are sparsely understood. To decipher the molecular functions of in Pi-homeostasis in rice, integrated transcriptome-proteome analysis of contrasting rice genotypes grown under Pi-starvation stress was performed. Our integrated analysis identified 4614 transcripts and 107 proteins differentially expressed in roots of NIL-23 (Pi-deficiency tolerant, harboring ), whereas 8555 transcripts and 687 proteins were differentially expressed in shoots of NIL-23 under Pi-starvation stress. The integrated analysis indicated 146 transcripts/proteins expressed in shoots, while 18 transcripts/proteins expressed in roots of NIL-23, which might be responsible for making it stress resilient. The genes/proteins for TFs, P metabolism, starch/sucrose metabolism, and RNA-mediated post-transcriptional regulation play important roles in Pi-starvation in rice. Functional annotation of differentially transcribed genes/expressed proteins indicated transcription factors, phosphorus/starch/sucrose metabolism, and RNA-mediated post-transcriptional regulation to be important players in Pi-starvation resilience of NIL-23. Up-regulated expression of genes/proteins under stress due to introgression of deciphered regulatory functions of the QTL and the genes/proteins involved. These might help developing rice cultivar with improved P acquisition/use-efficiency for better performance in P-deficient soils.

中文翻译：

---

近等基因系的转录组和蛋白质组综合分析为磷饥饿胁迫下水稻 Pup1 QTL 的调控功能提供了见解

磷是植物生长/生产力所必需的，通过根部以无机磷酸盐 (Pi) 的形式获取。植物耐低磷的分子机制尚不完全清楚。 QTL 在水稻 Pi 缺乏耐受性中的功能知之甚少。为了破译水稻中 Pi 稳态的分子功能，对 Pi 饥饿胁迫下生长的对比水稻基因型进行了转录组-蛋白质组综合分析。我们的综合分析确定了 NIL-23（耐 Pi 缺陷，隐匿）根中的 4614 个转录本和 107 个蛋白质差异表达，而在 Pi 饥饿胁迫下 NIL-23 的芽中有 8555 个转录本和 687 个蛋白质差异表达。综合分析表明，NIL-23 有 146 个转录本/蛋白质在芽中表达，而 18 个转录本/蛋白质在根中表达，这可能是使其具有逆境恢复能力的原因。转录因子、磷代谢、淀粉/蔗糖代谢和 RNA 介导的转录后调控的基因/蛋白质在水稻磷饥饿中发挥重要作用。差异转录基因/表达蛋白的功能注释表明转录因子、磷/淀粉/蔗糖代谢和RNA介导的转录后调控在NIL-23的Pi饥饿恢复中发挥重要作用。由于 QTL 和相关基因/蛋白质的破译调节功能的渗入，在应激下基因/蛋白质的表达上调。这些可能有助于开发提高磷获取/利用效率的水稻品种，从而在缺磷土壤中获得更好的表现。