Drought is a severe abiotic stress condition that impedes plant growth and development. During adaptation to drought conditions, plants release numerous small-molecule compounds that enhance their adaptive capacity. Pall. is an ecologically valuable plant; however, its metabolic adaptation mechanisms in different soil–water habitats have not been clearly reported. In this study, high-performance liquid chromatography (HPLC) and non-targeted metabolomic techniques were combined to investigate the growth adaptation mechanisms of in different soil environments. The results showed that the response of leaves to drought mainly manifested as a significant increase in abscisic acid (ABA) levels and a significant decrease in gibberellin (GA) levels (except during flowering). Drought led to the production of more metabolites during the growth and development of but reduced the complexity of the metabolite network. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed significant differences in the growth adaptation pathways of metabolites between the two habitats. In riparian habitats (RH), the phenylpropane biosynthesis pathway was significantly upregulated, while the synthesis of cutin, suberin, wax, α-linolenic acid metabolism, and linoleic acid metabolism was significantly downregulated. However, the tryptophan metabolism pathway was consistently downregulated in desert habitats (DH), with flavonoid and flavonol biosynthesis pathways playing important roles in drought resistance. The random forest model showed that the soil environment explained 3.21–94.34 % of the metabolite variation. Furthermore, structural equation modeling demonstrated that soil water had an indirect and positive effect on flavonoid metabolites by regulating ABA. This study revealed the adaptive mechanisms of hormones and metabolites in under drought conditions. It identified certain hormones that could potentially be harnessed for the development of plant additives, providing potential opportunities for exploring new stress adaptation strategies and developing new plant drought resistance products.

中文翻译：

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西伯利亚白刺在干旱中的生长适应：激素介导的类黄酮积累、代谢途径改变和网络复杂性降低

干旱是一种严重的非生物胁迫条件，阻碍植物生长和发育。在适应干旱条件的过程中，植物释放大量小分子化合物，增强其适应能力。颇尔。是一种具有生态价值的植物；然而，其在不同土壤-水生境中的代谢适应机制尚未明确报道。本研究结合高效液相色谱（HPLC）和非靶向代谢组学技术，研究不同土壤环境下的生长适应机制。结果表明，叶片对干旱的响应主要表现为脱落酸（ABA）水平显着升高，赤霉素（GA）水平显着下降（开花期除外）。干旱导致生长发育过程中产生更多代谢物，但降低了代谢物网络的复杂性。京都基因和基因组百科全书（KEGG）富集分析揭示了两个栖息地之间代谢物的生长适应途径存在显着差异。在河岸生境（RH）中，苯丙烷生物合成途径显着上调，而角质、木栓质、蜡的合成、α-亚麻酸代谢和亚油酸代谢显着下调。然而，色氨酸代谢途径在沙漠栖息地（DH）中持续下调，类黄酮和黄酮醇生物合成途径在抗旱中发挥着重要作用。随机森林模型表明，土壤环境解释了 3.21-94.34% 的代谢物变异。此外，结构方程模型表明，土壤水通过调节 ABA 对类黄酮代谢产生间接和积极的影响。这项研究揭示了干旱条件下激素和代谢物的适应机制。它确定了某些可能用于开发植物添加剂的激素，为探索新的胁迫适应策略和开发新的植物抗旱产品提供了潜在的机会。