Mineralized collagen scaffold is one of the best choices for bone defects treatment, but weak mechanical strength is the main factor restricting its development. Recent studies demonstrated that despite being a fundamental form of mechanical stimulation in human activities, the impact of cyclic compressive stress on collagen mineralization remains unclear, with even less known about the dynamic mechanical mechanism. This study employed cyclic compressive stress to investigate its effect on collagen mineralization. The findings revealed that cyclic compressive strain promotes collagen mineralization by facilitating increased mineral penetration into the collagen and altering mineral morphology on the collagen surface. As the mineral volume fraction of mineralized collagen rises, its elastic modulus also increases. Additionally, the finite element simulation results proved that cyclic compressive stress can impact mineral distribution by affecting their transport and deposition, consequently influencing the stress distribution and regulating mechanical properties of mineralized collagen. Alterations in mechanical properties provide feedback on internal stress distribution, subsequently impacting mineral mineralization. This study achieves a closed-loop study on the mechanical regulated collagen mineralization, offers insight into the mechanism of collagen mineralization, paving the way for further exploration of biomineralization mechanisms and potentially inspiring novel approaches for the fabrication of mineralized collagen scaffolds.

中文翻译：

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压应力通过动态调节矿化程度（一种闭环调节机制）来改善矿化胶原蛋白的机械性能

矿化胶原支架是骨缺损治疗的最佳选择之一，但机械强度较弱是制约其发展的主要因素。最近的研究表明，尽管循环压缩应力是人类活动中机械刺激的基本形式，但其对胶原蛋白矿化的影响仍不清楚，对其动态机械机制知之甚少。本研究采用循环压应力来研究其对胶原蛋白矿化的影响。研究结果表明，循环压缩应变通过促进矿物质渗透到胶原蛋白中并改变胶原蛋白表面的矿物质形态来促进胶原蛋白矿化。随着矿化胶原蛋白的矿物质体积分数的增加，其弹性模量也增加。此外，有限元模拟结果证明，循环压应力可以通过影响矿物的运输和沉积来影响矿物分布，从而影响应力分布并调节矿化胶原的力学性能。机械性能的变化提供有关内应力分布的反馈，从而影响矿物矿化。该研究实现了机械调节胶原蛋白矿化的闭环研究，深入了解胶原蛋白矿化机制，为进一步探索生物矿化机制铺平了道路，并可能启发制造矿化胶原蛋白支架的新方法。