In this study, a simple, specific and sensitive immunosensor for CEA detection was prepared based on BN nanosheets. Lewis acid-base interaction was sufficient for the immobilization of anti-CEA used as an antibody on the electrode surface without an activation agent. This immunosensor could be used for CEA determination without the need to use any label or secondary antibody. With its epedance-based recognition mechanism, this immunosensor offered a low LOD value of 0.017 ng/mL and a wide measurement range of 0.1–500 ng/mL and could be used for a long time. The analytical performance of this immunosensor is higher than the biosensors prepared in the literature. Compared to commercially available kits, it is attractive because it is cheap, simple and analyzes in a short time. This immunosensor, which has high selectivity against CEA in the presence of competitive agents in commercial human serum, has a high potential for clinical applications.

中文翻译：

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使用功能化氮化硼纳米片通过无标记电化学免疫传感器测定癌胚抗原（CEA）

本研究基于BN纳米片制备了一种简单、特异、灵敏的CEA检测免疫传感器。路易斯酸碱相互作用足以将用作抗体的抗 CEA 固定在电极表面上，无需活化剂。该免疫传感器可用于 CEA 测定，无需使用任何标记或二抗。凭借其基于阻抗的识别机制，该免疫传感器具有0.017 ng/mL的低LOD值和0.1-500 ng/mL的宽测量范围，并且可以长期使用。该免疫传感器的分析性能高于文献制备的生物传感器。与市售试剂盒相比，它很有吸引力，因为它便宜、简单且分析时间短。这种免疫传感器在商业人血清中存在竞争性药物的情况下对 CEA 具有高选择性，具有很高的临床应用潜力。