Coumarin, a secondary metabolite from the plant phenylpropane pathway, was certified as an important natural product for plants in response to abiotic stresses. Cytochrome P450s represent the largest monooxygenase superfamily in kingdoms, participating in various biochemical processes including coumarin biosynthesis. Here, we reported a total of 290 genes identified from the genome, and these were grouped into 9 clans. These genes were unevenly distributed in eight chromosomes and all of them contain highly conserved feature motifs of P450s. was differentially expressed in JiMa46 and JiMa49, meanwhile up-regulated in response to NaCl treatment. The heterologous expression in yeast showed that the transformed yeast cells exhibited obvious resistance under drought and salt stresses. Furthermore, overexpression of increased coumarin content in transgenic hairy roots. This study not only identified as an important regulator that adjusts drought and salt resistance, but also provided a link between coumarin metabolism and abiotic stresses.

中文翻译：

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细胞色素 P450 超家族的全基因组分析表明其在白草木樨香豆素生物合成和盐胁迫反应中的作用

香豆素是植物苯丙烷途径的次级代谢产物，被认证为植物应对非生物胁迫的重要天然产物。细胞色素 P450 代表王国中最大的单加氧酶超家族，参与包括香豆素生物合成在内的各种生化过程。在这里，我们报告了从基因组中鉴定出的总共 290 个基因，这些基因被分为 9 个家族。这些基因不均匀地分布在8条染色体上，并且都含有高度保守的P450特征基序。在 JiMa46 和 JiMa49 中差异表达，同时响应 NaCl 处理而上调。在酵母中的异源表达表明，转化的酵母细胞在干旱和盐胁迫下表现出明显的抗性。此外，转基因毛状根中香豆素含量的过度表达增加。这项研究不仅确定了香豆素是调节干旱和耐盐性的重要调节剂，而且还提供了香豆素代谢与非生物胁迫之间的联系。