The diversity, composition and performance of microbial communities within constructed wetlands (CW) were markedly influenced by spatio-temporal variations. A pilot-scale integrated vertical-flow constructed wetland (IVCW) as the biological purification unit within a recirculating aquaculture system (RAS) was established and monitored in this study. The investigation aimed to elucidate the responses of community structure, co-occurrence networks, and assembly mechanisms of the microbial community to spatial and temporal changes. Spatially, all -diversity indices and microbial networks complexity were significantly higher in the upstream pool of the IVCW than in the downstream pool. Temporally, the richness increased over time, while the evenness showed a decreasing trend. The number of nodes and edges of microbial networks increased over time. Notably, the stable pollutant removal efficiencies were observed during IVCW operations, despite -diversity and bacterial community networks exhibited significant variations across time. Functional redundancy emerged as a likely mechanism contributing to the stability of microbial ecosystem functions. Null model and neutral model analyses revealed the dominance of deterministic processes shaping microbial communities over time, with deterministic influences being more pronounced at lower -diversity levels. DO and inorganic nitrogen emerged as the principal environmental factor influencing microbial community dynamics. This study provides a theoretical foundation for the regulation of microbial communities and environmental factors within the context of IVCW.

中文翻译：

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基于循环水养殖系统的人工湿地微生物群落结构：探索时空变化和组装机制

人工湿地（CW）内微生物群落的多样性、组成和性能受到时空变化的显着影响。本研究建立并监测了一个中试规模的集成垂直流人工湿地（IVCW）作为循环水产养殖系统（RAS）内的生物净化单元。该研究旨在阐明微生物群落的群落结构、共现网络和组装机制对时空变化的响应。在空间上，IVCW上游池的全多样性指数和微生物网络复杂性显着高于下游池。从时间上看，丰富度随着时间的推移而增加，而均匀度则呈现下降的趋势。微生物网络的节点和边缘的数量随着时间的推移而增加。值得注意的是，尽管多样性和细菌群落网络随着时间的推移表现出显着变化，但在 IVCW 运行期间观察到稳定的污染物去除效率。功能冗余成为有助于微生物生态系统功能稳定性的一种可能机制。零模型和中性模型分析揭示了随着时间的推移塑造微生物群落的确定性过程的主导地位，确定性影响在较低多样性水平上更为明显。 DO 和无机氮成为影响微生物群落动态的主要环境因素。本研究为 IVCW 背景下微生物群落和环境因素的调控提供了理论基础。