As a tumor-suppressing protein, p53 plays a crucial role in preventing cancer development. Its utility as an early cancer detection tool is significant, potentially enabling clinicians to forestall disease advancement and improve patient prognosis. In response to the pathological overexpression of this antigen in tumors, the prevalence of anti-p53 antibodies increases in serum, in a manner quantitatively indicative of cancer progression. This spike can be detected through techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation. In this study, we present an electrochemical approach that supports ultrasensitive and highly selective anti-p53 autoantibody quantification without the use of an immuno-modified electrode. We specifically employ antigen-mimicking and antibody-capturing peptide-coated magnetic nanoparticles, along with an AC magnetic field-promoted sample mixing, prior to the presentation of Fab-captured targets to simple lectin-modified sensors. The subfemtomolar assays are highly selective and support quantification from serum-spiked samples within minutes.

中文翻译：

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超灵敏片上集成抗 p53 抗体定量中的抗原模拟纳米颗粒

作为一种肿瘤抑制蛋白，p53 在预防癌症发展中发挥着至关重要的作用。它作为早期癌症检测工具的效用非常重要，有可能使临床医生能够阻止疾病进展并改善患者预后。为了响应肿瘤中该抗原的病理性过度表达，血清中抗 p53 抗体的流行率增加，以定量指示癌症进展的方式。这种尖峰可以通过蛋白质印迹、免疫组织化学和免疫沉淀等技术来检测。在这项研究中，我们提出了一种电化学方法，支持超灵敏和高选择性的抗 p53 自身抗体定量，而无需使用免疫修饰电极。在将 Fab 捕获的靶标呈现给简单的凝集素修饰传感器之前，我们特别采用抗原模拟和抗体捕获肽包被的磁性纳米粒子，以及交流磁场促进的样品混合。亚飞摩尔检测具有高度选择性，支持在几分钟内对血清加标样品进行定量。