Fungal control strategies based on the use of have emerged in agriculture as eco-friendly alternatives to replace/reduce the use of synthetic pesticides. sp. P1 was reported as a new promising strain for control of a known producer of ochratoxin A, categorized as possible human carcinogen with high nephrotoxic potential. Grape quality can be influenced by vineyard management practices, including the use of fungal control agents. The aim of this study was to evaluate, for the first time, the quality parameters of Chardonnay grapes exposed to an antifungal -based strategy for control of , supporting findings by genomic investigations. Furthermore, genomic tools were used to confirm that the strain P1 belongs to the non-pathogenic species and also to certify its biosafety. The genome of P1 harbors genes that are putatively involved in the production of volatiles and hydrolytic enzymes, which are responsible for releasing the free form of aroma compounds. In addition to promote biocontrol of phytopathogenic fungi and ochratoxins, the treatment with P1 did not change the texture (hardness and firmness), color and pH of the grapes. Heat map and hierarchical clustering analysis (HCA) of volatiles evaluated by GC/MS revealed that -treated grapes showed higher levels of compounds with a pleasant odor descriptions such as 3-hydroxy-2-butanone, 2,3-butanediol, 3-methyl-1-butanol, 3,4-dihydro-β-ionone, β-ionone, dihydroactinidiolide, linalool oxide, and β-terpineol. The results of this study indicate that P1 presents desirable properties to be used as a biocontrol agent.

中文翻译：

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基因组分析揭示了编码基于贝莱斯芽孢杆菌的生物杀菌剂合成挥发性化合物的基因，该生物杀菌剂用于处理葡萄以控制碳曲霉

基于使用的真菌控制策略已在农业中出现，作为替代/减少合成农药使用的生态友好替代品。sp。据报道，P1 是一种新的有前途的菌株，可用于控制已知的赭曲霉毒素 A 生产者，赭曲霉毒素 A 被归类为具有高肾毒性潜力的可能人类致癌物。葡萄品质可能会受到葡萄园管理实践的影响，包括真菌控制剂的使用。本研究的目的是首次评估霞多丽葡萄的质量参数，该葡萄暴露于基于抗真菌策略的控制中，支持基因组研究的发现。此外，利用基因组工具确认菌株P1属于非致病菌种，并证明其生物安全性。P1 的基因组含有推测参与挥发物和水解酶产生的基因，这些酶负责释放游离形式的香气化合物。除了促进植物病原真菌和赭曲霉毒素的生物防治外，P1 处理不会改变葡萄的质地（硬度和硬度）、颜色和 pH 值。通过 GC/MS 评估的挥发物的热图和层次聚类分析 (HCA) 显示，经处理的葡萄显示出较高含量的具有令人愉快气味的化合物，如 3-羟基-2-丁酮、2,3-丁二醇、3-甲基-1-丁醇、3,4-二氢-β-紫罗兰酮、β-紫罗兰酮、二氢猕猴桃内酯、氧化芳樟醇和β-萜品醇。这项研究的结果表明，P1 具有用作生物防治剂的理想特性。