We developed a simulation model of human oral microbiota using Bio Palette oral medium (BPOM) containing 0.02% glucose and lower bacterial nitrogen sources, derived from saliva and dental plaque. By decreasing the concentration of Gifu anaerobic medium (GAM) from 30 to 10 g L−1, we observed increased ratios of target pathogenic genera, Porphyromonas and Fusobacterium from 0.5% and 1.7% to 1.2% and 3.5%, respectively, in the biofilm on hydroxyapatite (HA) discs. BPOM exhibited the higher ratios of Porphyromonas and Fusobacterium, and amplicon sequence variant number on HA, compared with GAM, modified GAM and basal medium mucin. Mixing glycerol stocks of BPOM culture solutions from four human subjects resulted in comparable ratios of these bacteria to the original saliva. In this simulation model, sitafloxacin showed higher inhibitory effects on P. gingivalis than minocycline hydrochloride at a low dosage of 0.1 μg mL−1. Probiotics such as Streptococcus salivarius and Limosilactobacillus fermentum also showed significant decreases in Porphyromonas and Fusobacterium ratios on HA, respectively. Overall, the study suggests that BPOM with low carbon and nutrients could be a versatile platform for assessing the efficacy of antibiotics and live biotherapeutics in treating oral diseases caused by Porphyromonas and Fusobacterium.

中文翻译：

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利用人类口腔微生物群构建多功能体外培养筛选平台

我们使用含有 0.02% 葡萄糖和来自唾液和牙菌斑的较低细菌氮源的 Bio Palette 口腔培养基 (BPOM) 开发了人类口腔微生物群的模拟模型。将岐阜厌氧培养基 (GAM) 的浓度从 30 g·L 降低至 10 g·L−1，我们观察到目标致病属的比例增加，卟啉单胞菌属和梭杆菌属羟基磷灰石 (HA) 盘上的生物膜中的含量分别从 0.5% 和 1.7% 降低到 1.2% 和 3.5%。BPOM 表现出较高的比率卟啉单胞菌属和梭杆菌属和 HA 上的扩增子序列变异数，与 GAM、修饰的 GAM 和基础培养基粘蛋白相比。将来自四名受试者的 BPOM 培养液的甘油原液混合后，这些细菌与原始唾液的比例相当。在此模拟模型中，西他沙星对牙龈卟啉单胞菌0.1 μg·mL 低剂量时优于盐酸米诺环素−1。益生菌如唾液链球菌和发酵柠檬酸乳杆菌也显示出显着下降卟啉单胞菌属和梭杆菌属分别为HA的比率。总体而言，该研究表明，具有低碳和营养成分的 BPOM 可以成为评估抗生素和活生物治疗药物治疗口腔疾病的功效的多功能平台。卟啉单胞菌属和梭杆菌属。