Early monitoring of MRSA can effectively mitigate the disease risk by using Penicillin-binding protein 2a (PbP2a) biomarker. Diamino naphthalene-AuNPs decorated graphene (AuNPsGO-DN) nanocomposite was synthesized for a rapid and sensitive immunosensor detecting PbP2a. The synthesized AuNPsGO-DN nanocomposites were characterized by field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (SEM-EDX), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, and X-ray diffraction spectroscopy (XRD). Electrochemical characterization done with cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrical impedance spectroscopy (EIS) techniques. Anti-PbP2a monoclonal antibodies immobilized at AuNPsGO-DN/GCE via covalent bonding. AuNPs enhanced the electrode surface area and the antibodies' loading. Mercaptopropionic acid (MPA) was a linker between the AuNPs and antibodies, orientated the antibodies as opposite to the PbP2a antigen, and improved the sensitivity and specificity. The antiPbP2a/MPA/AuNPsGO-DN/GCE electrode displayed sensitive and selective detection towards the PbP2a antigen in phosphate buffer saline (PBS pH 7.4). The broad linear range from 0.01 to 8000 pg/mL was obtained with LOD of 0.154 pg/mL and 0.0239 pg/mL, respectively. A label-free, simple, and sensitive immunosensor was developed with a 98–106 % recovery rate in spiked biological samples. It shows the potential applicability of the developed immunoelectrode.

中文翻译：

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一锅法制备二氨基萘-AuNPs装饰石墨烯纳米平台用于生物样品中的MRSA检测

通过使用青霉素结合蛋白2a（PbP2a）生物标志物，早期监测MRSA可以有效降低疾病风险。合成了二氨基萘-AuNPs 修饰的石墨烯 (AuNPsGO-DN) 纳米复合材料，用于快速灵敏地检测 PbP2a 的免疫传感器。通过场发射扫描电子显微镜 (FE-SEM)、能量色散 X 射线光谱 (SEM-EDX)、傅里叶变换红外光谱 (FT-IR)、拉曼光谱和 X 射线对合成的 AuNPsGO-DN 纳米复合材料进行了表征。衍射光谱（XRD）。使用循环伏安法 (CV)、微分脉冲伏安法 (DPV) 和电阻抗光谱 (EIS) 技术进行电化学表征。抗 PbP2a 单克隆抗体通过共价键固定在 AuNPsGO-DN/GCE 上。 AuNPs 增强了电极表面积和抗体负载量。巯基丙酸 (MPA) 是 AuNP 和抗体之间的连接体，使抗体与 PbP2a 抗原相反，并提高了灵敏度和特异性。 antiPbP2a/MPA/AuNPsGO-DN/GCE 电极对磷酸盐缓冲盐水（PBS pH 7.4）中的 PbP2a 抗原表现出灵敏和选择性的检测。 LOD 分别为 0.154 pg/mL 和 0.0239 pg/mL，获得了 0.01 至 8000 pg/mL 的宽线性范围。开发了一种无标记、简单且灵敏的免疫传感器，在加标生物样品中回收率为 98-106%。它显示了所开发的免疫电极的潜在适用性。