The pyrabactin resistance (PYR)/PYR-like (PYL)/regulatory component of ABA receptor (RCAR) proteins are the core components in ABA signaling pathway. A salt and cold induced ABA receptor gene from common vetch was identified in the present study. Transgenic overexpressing had increased ABA sensitivity and higher levels of seed germination rate, survival rate, and the maximal photochemical efficiency of photosystem II () than the wild type (WT) under salinity conditions. Lower levels of the temperature that leads to 50% electrolyte leakage (TEL) and higher levels of survival rate after freezing treatment were observed in transgenic lines compared with WT. The results indicated that regulated salt and cold tolerance positively. Lower levels of Na and Na/K ratio but higher levels of K and , , , and transcripts were observed in transgenic lines compared with WT under salinity conditions. The results indicated that the increased salt tolerance in transgenic lines was associated with the altered Na and K levels as a result of improved expression of genes involved in Na and K homeostasis. Antioxidant enzyme activities and their encoding gene transcripts, proline concentration and reactive oxygen species (ROS) levels were increased after treatment with salinity or cold, with lower levels in transgenic lines compared with WT. It is suggested that -regulated salt and cold tolerance was associated with the increased proline level and antioxidant defense capacity for maintenance of ROS as a result of improved the relevant gene expression under salinity and cold conditions. Overexpression of VsPYL5 led to increased salt and cold tolerance through improved expression of genes involved in Na and K homeostasis and ROS homeostasis and increased antioxidant defense capacity

中文翻译：

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普通野豌豆ABA受体基因VsPYL5的过表达增强拟南芥的耐盐性和耐寒性

吡拉巴菌素抗性(PYR)/PYR样(PYL)/ABA受体调节成分(RCAR)蛋白是ABA信号通路的核心成分。本研究鉴定了来自普通野豌豆的盐和冷诱导的 ABA 受体基因。在盐度条件下，转基因过表达比野生型（WT）提高了ABA敏感性，并且种子发芽率、存活率和光系统II（）的最大光化学效率水平更高。与野生型相比，转基因品系中观察到导致 50% 电解质渗漏 (TEL) 的温度较低，冷冻处理后的存活率较高。结果表明，对盐和耐冷能力有积极的调节作用。在盐度条件下，与野生型相比，转基因品系中观察到 Na 和 Na/K 比率水平较低，但 K 和 、 、 、 和转录物水平较高。结果表明，转基因品系中耐盐性的增加与钠和钾水平的改变有关，这是由于涉及钠和钾稳态的基因表达改善所致。经盐度或寒冷处理后，抗氧化酶活性及其编码基因转录本、脯氨酸浓度和活性氧（ROS）水平均有所增加，但与野生型相比，转基因品系中的水平较低。这表明，盐和冷耐受性调节与脯氨酸水平和维持ROS的抗氧化防御能力的增加有关，这是由于盐度和寒冷条件下相关基因表达的改善而导致的。 VsPYL5 的过度表达通过改善涉及 Na 和 K 稳态以及 ROS 稳态的基因表达来提高耐盐性和耐冷性，并提高抗氧化防御能力