As mitochondrial damage or dysfunction is commonly observed following burn injuries, we investigated whether mitochondrial transplantation (MT) can result in therapeutic benefits in the treatment of burns. Human immortalized epidermal cells (HaCaT) and Kunming mice were used to establish a heat-injured cell model and a deep partial-thickness skin burn animal model, respectively. The cell model was established by exposing HaCaT cells to 45 or 50 °C for 10 min, after which cell proliferation was assayed using fluorescent double-staining and colony formation assays, cell migration was assessed using colloidal gold migration and scratch assays, and cell cycle progression and apoptosis were measured by flow cytometry. Histopathological staining, immunohistochemistry, nick-end labeling analysis, and enzyme-linked immunosorbent assays were used to evaluate the effects of MT on inflammation, tissue recovery, apoptosis, and scar growth in a mouse model. The therapeutic effects were observed in the heat-injured HaCaT cell model. MT promoted cell viability, colony formation, proliferation, and migration; decreased G1 phase; promoted cell division; and decreased apoptosis. Wound-healing promotion, anti-inflammation (decreased mast cell aggregation, down-regulated of TNF-α, IL-1β, IL-6, and up-regulated IL-10), acceleration of proliferation recovery (up-regulated CD34 and VEGF), apoptosis reduction, and scar formation reduction (decreased collagen I/III ratio and TGF-β1) were observed in the MT mouse model. The MT mode of action was, however, not investigated in this study. In conclusion, our data indicate that MT exerts a therapeutic effect on burn injuries both and .

中文翻译：

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线粒体移植对烧伤的治疗作用

由于烧伤后通常会观察到线粒体损伤或功能障碍，因此我们研究了线粒体移植（MT）是否可以在烧伤治疗中产生治疗效果。采用人永生化表皮细胞（HaCaT）和昆明小鼠分别建立热损伤细胞模型和深部皮肤烧伤动物模型。通过将HaCaT细胞暴露于45或50°C 10分钟来建立细胞模型，然后使用荧光双染和集落形成测定来测定细胞增殖，使用胶体金迁移和划痕测定来评估细胞迁移，以及细胞周期通过流式细胞术测量进展和凋亡。使用组织病理学染色、免疫组织化学、缺口末端标记分析和酶联免疫吸附测定来评估 MT 对小鼠模型炎症、组织恢复、细胞凋亡和疤痕生长的影响。在热损伤 HaCaT 细胞模型中观察治疗效果。 MT 促进细胞活力、集落形成、增殖和迁移； G1期减少；促进细胞分裂；并减少细胞凋亡。促进伤口愈合、抗炎（减少肥大细胞聚集、下调TNF-α、IL-1β、IL-6和上调IL-10）、加速增殖恢复（上调CD34和VEGF在 MT 小鼠模型中观察到细胞凋亡减少和疤痕形成减少（胶原蛋白 I/III 比例和 TGF-β1 降低）。然而，本研究并未研究 MT 的作用模式。总之，我们的数据表明 MT 对烧伤和 烧伤均具有治疗作用。