Deubiquitinating enzymes (DUBs) are essential for plant responses to abiotic stress. However, the function of these genes in , an ideal woody plant species for investigating abiotic stress response mechanisms, is largely unknown. In our previous study, a novel gene, , was revealed to contribute to the resistance of seeds to salinity stress In the current study, was cloned and functionally characterized. A phylogenetic analysis indicated PeMINDY3 belongs to the MINDY subfamily of DUBs. Subcellular localization results showed that PeMINDY3 is located in chloroplasts. According to the qRT-PCR and GUS staining analyses, expression was induced in response to drought and high salinity treatment. The ectopic expression of in increased the tolerance to drought and salt stress. Moreover, compared with the wild-type (WT) plants, plants ectopically expressing had longer primary roots and higher fresh weights, relative water contents, and antioxidant enzyme activities, but lower relative electrical conductivities. Additionally, stress-related genes were more highly expressed in the ectopically expressing plants than in the WT plants after the abiotic stress treatments. Furthermore, yeast one-hybrid and luciferase assays identified PeRAV2 as a regulator of expression. These findings suggest that positively modulates drought and salt stress tolerance, providing a foundation for further exploration of the function of .

中文翻译：

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一种新的胡杨 DUB 基因 PeMINDY3 通过促进 ROS 清除来增强耐旱和耐盐能力

去泛素化酶 (DUB) 对于植物应对非生物胁迫至关重要。然而，这些基因在研究非生物胁迫反应机制的理想木本植物物种中的功能在很大程度上尚不清楚。在我们之前的研究中，一个新的基因，被发现有助于种子对盐胁迫的抵抗力。在当前的研究中，该基因被克隆并进行了功能表征。系统发育分析表明 PeMINDY3 属于 DUB 的 MINDY 亚家族。亚细胞定位结果表明PeMINDY3定位于叶绿体。根据 qRT-PCR 和 GUS 染色分析，表达是针对干旱和高盐度处理而诱导的。 in的异位表达增加了对干旱和盐胁迫的耐受性。此外，与野生型（WT）植物相比，异位表达的植物具有更长的初生根和更高的鲜重、相对含水量和抗氧化酶活性，但相对电导率较低。此外，非生物胁迫处理后，与野生型植物相比，异位表达植物中胁迫相关基因的表达更高。此外，酵母单杂交和荧光素酶测定确定 PeRAV2 是表达调节因子。这些发现表明，积极调节干旱和盐胁迫耐受性，为进一步探索 的功能奠定了基础。