N-methyladenosine (mA) is a post-transcriptional modification found in eukaryotic mRNAs. It is installed and removed by methyltransferases and demethylases, respectively. Recent studies have demonstrated that alpha-ketoglutarate-dependent dioxygenase homolog 10B (ALKBH10B) is an mRNA mA eraser affecting floral transition and responding to abiotic stresses in . However, the roles of mA eraser proteins, especially cotton GhALKBH10B, in plant adaptation to abiotic stresses are largely unknown. In this study, we aimed to determine the role of GhALKBH10B in the response of cotton to drought stress. The mA level significantly increased in -silenced plants compared to that in the control plants. Seedling growth and survival rate of -silenced plants were enhanced under drought stress, while heterologous expression of in attenuates the drought tolerance. Notably, drought-tolerant phenotypes of -silenced plants were correlated with the increased mRNA stability of photosynthesis-related genes and which is a key regulator in ABA-dependent pathway under both normal and dehydration conditions. In addition, GhALKBH10B interacts with a RNA binding protein GhSERBP1(Hyaluronic / mRNA binding family HABP4_PAI-RBP1) both and and silencing decreased the drought tolerance in cotton. Collectively, these findings indicate that -mediated mA demethylation affects the transcript levels of photosynthesis-related genes and which are important for survival of cotton in response to drought stress. This will provide important clues for revealing the novel molecular mechanism at the epi-transcriptomic level in drought response and provide key candidate genes for cotton drought-tolerant breeding in the future.

中文翻译：

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GhALKBH10B 的下调通过提高棉花光合作用相关和 ABA 信号通路基因的稳定性来提高耐旱性

N-甲基腺苷 (mA) 是真核 mRNA 中发现的转录后修饰。它分别由甲基转移酶和去甲基酶安装和去除。最近的研究表明，α-酮戊二酸依赖性双加氧酶同源物 10B (ALKBH10B) 是一种 mRNA m6A 擦除器，影响花的转变并响应非生物胁迫。然而，m6A擦除蛋白，尤其是棉花GhALKBH10B，在植物适应非生物胁迫中的作用尚不清楚。在本研究中，我们旨在确定 GhALKBH10B 在棉花响应干旱胁迫中的作用。与对照植物相比，β-沉默植物中的mA水平显着增加。干旱胁迫下α沉默植物的幼苗生长和成活率得到提高，而α的异源表达则减弱了其耐旱性。值得注意的是，沉默植物的耐旱表型与光合作用相关基因的 mRNA 稳定性增加相关，并且光合作用相关基因是正常和脱水条件下 ABA 依赖性途径的关键调节因子。此外，GhALKBH10B 与 RNA 结合蛋白 GhSERBP1（透明质酸/mRNA 结合家族 HABP4_PAI-RBP1）相互作用，并且沉默会降低棉花的耐旱性。总的来说，这些发现表明，m6A去甲基化会影响光合作用相关基因的转录水平，这对于棉花应对干旱胁迫的生存很重要。这将为揭示干旱响应的表观转录组新分子机制提供重要线索，并为未来棉花耐旱育种提供关键候选基因。