Cryomicroneedles (cryoMNs) offer a convenient and minimally invasive way to precisely deliver therapeutic cells intradermally for treating local and systemic diseases. cryoMNs are manufactured by shaping and freezing the cell-containing cryogenic media in a microneedle template, which allows cells to be packaged in advance for direct usage in the clinic. However, the current cryoMNs require cold-chain transportation and storage and do not permit the loading of autologous cells in situ. This article introduces the second generation of cryoMNs (S-cryoMNs) that address these limitations. Specifically, S-cryoMNs are made by dipping a porous MN scaffold in the cell suspension before cryopreservation. The porous scaffold can be transported at room temperature, and researchers can load any cells with the optimized cryogenic medium. As a proof-of-concept, we examined the loading and intradermal delivery of three cell types in clinically relevant in vitro and in vivo models, including mesenchymal stem cells for wound healing, melanocytes for vitiligo treatment, and antigen-pulsed dendritic cells for cancer vaccination.

冷冻微针 (cryoMN) 提供了一种方便且微创的方法，可以在皮内精确输送治疗细胞，以治疗局部和全身疾病。CryoMN 是通过在微针模板中成型和冷冻含有细胞的低温介质来制造的，这使得细胞可以提前包装以便在临床中直接使用。然而，目前的cryoMN需要冷链运输和储存，并且不允许原位装载自体细胞。本文介绍了解决这些限制的第二代 CryoMN (S-cryoMN)。具体来说，S-cryoMN 是通过在冷冻保存前将多孔 MN 支架浸入细胞悬浮液中制成的。多孔支架可以在室温下运输，研究人员可以用优化的低温介质装载任何细胞。作为概念验证，我们在临床相关的体外和体内模型中检查了三种细胞类型的装载和皮内递送，包括用于伤口愈合的间充质干细胞、用于白癜风治疗的黑素细胞和用于癌症的抗原脉冲树突细胞疫苗接种。