Bone grafting is a fundamental dental surgery procedure widely used for implant placement and periodontal disease management treatments. Despite its broad applications, vertical bone augmentation presents unique challenges, including the risk of graft displacement due to gravitational and masticatory forces. Traditional physical stabilization methods introduce additional complexities and risks, underscoring the need for innovative fixation technologies. This study aimed to develop an in situ photo-crosslinkable bioadhesive hydrogel (iPBAH) as a multifunctional bone graft binder to enhance the process of bone reconstruction. The bioadhesive is composed of mussel-derived adhesive protein (MAP) fused with the cell-adhesive peptide RGD. The numerous tyrosine residues in MAP facilitate rapid photo-crosslinking, enabling efficient hydrogel formation using visible blue light. Subsequently, iPBAH underwent comprehensive characterization to evaluate its suitability as a multifunctional bone graft binder. iPBAH efficiently underwent in situ crosslinking through harmless exposure to visible light within minutes and displayed several exceptional properties, including a microporous structure, underwater adhesion, extended durability, high compressive strength, and biocompatibility. In vivo assessments, using male Sprague-Dawley rats, demonstrated that iPBAH binder significantly enhanced bone regeneration in a rat calvarial bone defect model. The in situ crosslinking of the iPBAH binder during bone graft transplantation can effectively fill irregular and complex defect shapes while simultaneously preventing graft material leakage. The improved physical attributes of the bound graft material can enhance its resistance to external forces, thereby ensuring sustained retention over time. Moreover, the interaction between iPBAH and surrounding tissues promotes adhesion and integration of the graft material with host tissues in the defect area. In addition, the included RGD peptide in iPBAH can augment inherent cell recruitment, adhesion, and growth, consequently expediting osteogenesis.

中文翻译：

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用于骨移植固定的原位光交联蛋白质生物粘合剂

骨移植是一种基本的牙科手术程序，广泛用于种植体植入和牙周病管理治疗。尽管应用广泛，但垂直骨增量提出了独特的挑战，包括由于重力和咀嚼力而导致移植物移位的风险。传统的物理稳定方法引入了额外的复杂性和风险，凸显了对创新固定技术的需求。本研究旨在开发一种原位光交联生物粘附水凝胶（iPBAH）作为多功能骨移植粘合剂，以增强骨重建过程。该生物粘附剂由贻贝来源的粘附蛋白 (MAP) 与细胞粘附肽 RGD 融合而成。MAP 中的大量酪氨酸残基促进快速光交联，从而利用可见蓝光实现有效的水凝胶形成。随后，对 iPBAH 进行了全面表征，以评估其作为多功能骨移植粘合剂的适用性。iPBAH 通过无害地暴露在可见光下，在几分钟内有效地进行原位交联，并显示出多种优异的性能，包括微孔结构、水下粘附、延长的耐用性、高压缩强度和生物相容性。使用雄性 Sprague-Dawley 大鼠进行的体内评估表明，iPBAH 粘合剂可显着增强大鼠颅骨骨缺损模型中的骨再生。iPBAH粘合剂在骨移植过程中的原位交联可以有效填充不规则和复杂的缺损形状，同时防止移植材料渗漏。结合的移植材料的物理属性得到改善，可以增强其对外力的抵抗力，从而确保随着时间的推移持续保持。此外，iPBAH 与周围组织之间的相互作用促进了移植材料与缺损区域宿主组织的粘附和整合。此外，iPBAH 中包含的 RGD 肽可以增强固有的细胞募集、粘附和生长，从而加速成骨。