Members of the Bacillus genus are widely distributed throughout natural environments and have been studied for decades among others for their physiology, genetics, ecological functions, and applications. However, despite its prevalence in nature, the characterization and classification of Bacillus remain challenging due to its complex and ever-evolving taxonomic framework. This review addresses the current state of the Bacillus taxonomic landscape and summarizes the critical points in the development of Bacillus phylogeny. With a clear view of Bacillus phylogeny as a foundation, we subsequently review the methodologies applied in identifying and quantifying Bacillus, while also discussing their respective advantages and disadvantages.

中文翻译：

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如何识别和定量芽孢杆菌属成员？

芽孢杆菌属的成员广泛分布在整个自然环境中，并且对其生理学、遗传学、生态功能和应用等方面的研究已有数十年之久。然而，尽管芽孢杆菌在自然界中普遍存在，但由于其复杂且不断发展的分类框架，芽孢杆菌的表征和分类仍然具有挑战性。这篇综述讨论了芽孢杆菌分类学景观的现状，并总结了芽孢杆菌系统发育发展的关键点。以芽孢杆菌系统发育的清晰观点为基础，我们随后回顾了用于鉴定和定量芽孢杆菌的方法，同时还讨论了它们各自的优点和缺点。