This study explored supercritical fluid extraction (SFE) of bioactive molecules from seeds, a little-known source of potent bioactive compounds. Response surface methodology assessed extraction parameters (pressure, temperature, and ethanol co-solvent) and their effects on global yield, total phenolic compound (TPC), total carbohydrate content (TCC), and anti-Alzheimer activity. Optimization via a Box-Behnken design identified the optimal conditions: 15% ethanol, 300bar, and 70°C. Chemical profiling using GC-MS and UHPLC-q-tof-MS/MS detected over 41 metabolites. In addition, a biorefinery sequential extraction process was performed to obtain extracts with high neuroprotective potential. The SFE extracts exhibited modest AChE inhibition. Notably, sequential SFE and PLE extractions yielded extracts with enhanced antioxidant activity compared to separate processes. In conclusion, presents a promising natural source of secondary metabolites. Its potential health benefits warrant further exploration, emphasizing the need for continued research.

中文翻译：

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多元响应面法优化超临界流体萃取白毛蝽果实生物活性化合物

这项研究探索了从种子中提取生物活性分子的超临界流体萃取（SFE），种子是一种鲜为人知的有效生物活性化合物的来源。响应面方法评估了提取参数（压力、温度和乙醇共溶剂）及其对总产量、总酚化合物（TPC）、总碳水化合物含量（TCC）和抗阿尔茨海默病活性的影响。通过 Box-Behnken 设计进行优化确定了最佳条件：15% 乙醇、300bar 和 70°C。使用 GC-MS 和 UHPLC-q-tof-MS/MS 进行化学分析，检测到超过 41 种代谢物。此外，还进行了生物精炼连续提取过程，以获得具有高神经保护潜力的提取物。SFE 提取物表现出适度的 AChE 抑制作用。值得注意的是，与单独的工艺相比，连续 SFE 和 PLE 提取所产生的提取物具有增强的抗氧化活性。总之，提出了一种有前途的次生代谢物天然来源。其潜在的健康益处值得进一步探索，强调需要继续研究。