Microbial activity in stored rice grains may induce quality deterioration and mycotoxin accumulation, resulting in significant economic losses and food safety risks. However, few studies have investigated the migration of microbial communities with storage time and space in stored rice grains. The aim of this study was to understand the assembly and changes of bacterial and fungal communities in rice grains during storage stages. A total of 108 rice samples were collected from 6 different depots, 2 storage times (1-year and 2-year), 3 vertical depths, and 3 horizontal positions during storage periods, and 16 S rRNA and ITS high-throughput sequencing analysis were performed. The main factors influencing the alpha diversity of bacteria and fungi in stored rice grains were grain depot and storage time. A total of 270 bacterial genera and 173 fungal genera were detected in all samples. The most dominant fungi species in all stored rice grains sample were (53.24%), (6.19%), (4.71%), (4.46%). The relative abundance of significantly decreased with the storage time increasing, while that of significantly increased. Collectively, this study will improve knowledge regarding microbial communities in rice grains during storage.

中文翻译：

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稻谷贮藏期间细菌和真菌群落的时空变化

储藏米粒中的微生物活动可能导致品质恶化和霉菌毒素积累，造成重大经济损失和食品安全风险。然而，很少有研究调查储藏米粒中微生物群落随储藏时间和空间的迁移情况。本研究的目的是了解稻米在储存阶段的细菌和真菌群落的组装和变化。共采集6个不同库房、2个储藏时间（1年和2年）、3个垂直深度、3个水平位置的水稻样品108份，进行16S rRNA和ITS高通量测序分析。执行。影响贮藏稻米细菌和真菌α多样性的主要因素是粮库和贮藏时间。所有样品中总共检测到270个细菌属和173个真菌属。所有储藏米粒样品中最主要的真菌种类分别为（53.24%）、（6.19%）、（4.71%）、（4.46%）。随着保存时间的延长， 的相对丰度显着降低，而 的相对丰度则显着增加。总的来说，这项研究将提高对储存期间稻谷微生物群落的认识。