Smart carrier-based immobilization has widened the use of enzymes for the treatment of several disorders. Large surface areas, tunable morphology, and surface modification ability aid the targeted and controlled release of therapeutic enzymes from such formulations. Smart nanocarriers, such as polymeric carriers, liposomes, and silica have also increased the stability, half-life, and permeability of these enzymes. In this review, summarize recent advances in the smart immobilization of microbial enzymes and their development as precision nanomedicine for the treatment of cancer, thrombosis, phenylketonuria (PKU), and wound healing. We also discuss the challenges and measures to be adopted for the successful clinical translation of these formulations.

中文翻译：

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用于治疗应用的基于智能纳米载体的微生物酶的靶向递送的最新趋势

基于智能载体的固定化扩大了酶在多种疾病治疗中的用途。大表面积、可调节的形态和表面修饰能力有助于治疗酶从此类制剂中的靶向和受控释放。智能纳米载体，例如聚合物载体、脂质体和二氧化硅，也提高了这些酶的稳定性、半衰期和渗透性。在这篇综述中，总结了微生物酶智能固定化的最新进展及其作为治疗癌症、血栓形成、苯丙酮尿症 (PKU) 和伤口愈合的精密纳米医学的发展。我们还讨论了这些制剂成功临床转化所面临的挑战和采取的措施。