Postoperative pancreatic leakage due to pancreatitis in patients is a life-threatening surgical complication. The majority of commercial barriers are unable to meet the demands for pancreatic leakage due to poor adhesiveness, toxicity, and inability to degrade. In this study, we fabricated mitomycin-c and thrombin-loaded multifunctional dual-layer nanofibrous membrane with a combination of alginate, PCL, and gelatin to resolve the leakage due to suture line disruption, promote hemostasis, wound healing, and prevent postoperative tissue adhesion. Electrospinning was used to fabricate the dual-layer system. The study results demonstrated that high gelatin and alginate content in the inner layer decreased the fiber diameter and water contact angle, and crosslinking allowed the membrane to be more hydrophilic, making it highly biodegradable, and adhering firmly to the tissue surfaces. The results of in vitro biocompatibility and hemostatic assay revealed that the dual-layer had a higher cell proliferation and showed effective hemostatic properties. Moreover, the in vivo studies and in silico molecular simulation indicated that the dual layer was covered at the wound site, prevented suture disruption and leakage, inhibited hemorrhage, and reduced postoperative tissue adhesion. Finally, the study results proved that dual-layer multifunctional nanofibrous membrane has a promising therapeutic potential in preventing postoperative pancreatic leakage.

中文翻译：

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多功能双层纳米纤维膜预防术后胰漏

胰腺炎患者术后胰漏是危及生命的手术并发症。大多数商业屏障由于粘附性差、毒性和无法降解而无法满足胰漏的要求。在本研究中，我们结合海藻酸盐、PCL和明胶制备了丝裂霉素-C和凝血酶负载的多功能双层纳米纤维膜，以解决因缝合线破坏引起的渗漏，促进止血、伤口愈合并防止术后组织粘连。静电纺丝用于制造双层系统。研究结果表明，内层的高明胶和海藻酸盐含量降低了纤维直径和水接触角，交联使膜更加亲水，使其具有高度生物降解性，并牢固地粘附在组织表面。体外生物相容性和止血实验结果表明，双层具有较高的细胞增殖能力，并表现出有效的止血性能。此外，体内研究和计算机分子模拟表明，双层覆盖伤口部位，防止缝线破裂和渗漏，抑制出血，并减少术后组织粘连。最后，研究结果证明双层多功能纳米纤维膜在预防术后胰漏方面具有良好的治疗潜力。