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A Microflow Cytometer Enabled by Monolithic Integration of a Microreflector with an Acoustic Resonator
ACS Sensors ( IF 8.2 ) Pub Date : 2024-02-21 , DOI: 10.1021/acssensors.3c02530 Yaping Wang 1 , Wei Wei 1 , Xieruiqi Guan 1 , Yang Yang 1 , Bingyi Tang 1 , Wenlan Guo 1 , Chen Sun 1 , Xuexin Duan 1
ACS Sensors ( IF 8.2 ) Pub Date : 2024-02-21 , DOI: 10.1021/acssensors.3c02530 Yaping Wang 1 , Wei Wei 1 , Xieruiqi Guan 1 , Yang Yang 1 , Bingyi Tang 1 , Wenlan Guo 1 , Chen Sun 1 , Xuexin Duan 1
Affiliation
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Current microflow cytometers suffer from complicated fluidic integration and low fluorescence collection efficiency, resulting in reduced portability and sensitivity. Herein, we demonstrated a new flow cell design based on an on-chip monolithically integrated microreflector with a bulk acoustic wave resonator (MBAW). It enables simultaneous 3D particle focusing and fluorescence enhancement without using shear flow. Benefited by the on-chip microreflector, the captured fluorescence intensity was 1.8-fold greater than that of the Si substrate and 8.3-fold greater than that of the SiO2 substrate, greatly improving the detection sensitivity. Combined with the contactless acoustic streaming-based focusing, particle sensing with a coefficient of variation as low as 6.1% was achieved. We also demonstrated the difference between live and dead cells and performed a cell cycle assay using the as-developed microflow cytometry. This monolithic integrated MBAW provides a new type of opto-acoustofluidic system and has the potential to be a highly integrated, highly sensitive flow cytometer for applications such as in vitro diagnostics and point of care.
中文翻译：
通过微反射器与声学谐振器的单片集成实现的微流式细胞仪
目前的微流式细胞仪存在复杂的流体集成和低荧光收集效率，导致便携性和灵敏度降低。在此，我们展示了一种基于带有体声波谐振器（MBAW）的片上单片集成微反射器的新型流通池设计。它无需使用剪切流即可同时进行 3D 粒子聚焦和荧光增强。得益于片上微反射器，捕获的荧光强度比Si基板高1.8倍，比SiO 2基板高8.3倍，大大提高了检测灵敏度。结合非接触式声流聚焦，实现了变异系数低至 6.1% 的颗粒传感。我们还证明了活细胞和死细胞之间的差异，并使用所开发的微流式细胞术进行了细胞周期测定。这种单片集成 MBAW 提供了一种新型光声流体系统，并有潜力成为高度集成、高灵敏度的流式细胞仪，适用于体外诊断和护理点等应用。
更新日期：2024-02-21
中文翻译：
通过微反射器与声学谐振器的单片集成实现的微流式细胞仪
目前的微流式细胞仪存在复杂的流体集成和低荧光收集效率，导致便携性和灵敏度降低。在此，我们展示了一种基于带有体声波谐振器（MBAW）的片上单片集成微反射器的新型流通池设计。它无需使用剪切流即可同时进行 3D 粒子聚焦和荧光增强。得益于片上微反射器，捕获的荧光强度比Si基板高1.8倍，比SiO 2基板高8.3倍，大大提高了检测灵敏度。结合非接触式声流聚焦，实现了变异系数低至 6.1% 的颗粒传感。我们还证明了活细胞和死细胞之间的差异，并使用所开发的微流式细胞术进行了细胞周期测定。这种单片集成 MBAW 提供了一种新型光声流体系统，并有潜力成为高度集成、高灵敏度的流式细胞仪，适用于体外诊断和护理点等应用。
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