Garlic essential oil (GEO) in water nanoemulsion (GEON) has been reported to possess good stability and broad-spectrum antibacterial activity, but its effect on is rarely studied. In the present study, the antibacterial, anti-biofilm formation activity and underlying mechanism of GEON against were investigated. Results showed that GEON (mean particle size = 136.43 nm) significantly inhibited the growth of planktonic and the minimum inhibitory concentration (MIC) of GEO in the nanoemulsion was 512 μg/mL. GEON could effectively kill in a time- and concentration-dependent manner. After GEON treatment, the cell membrane was severely damaged, which was proven by remarkable membrane depolarization and considerable loss of intracellular substances (nucleic acid, protein, and ATP). Moreover, decreased membrane integrity and collapsed cell morphology further confirmed the cell membrane damage. Additionally, GEON significantly attenuated the biofilm formation, enhanced the surface hydrophobicity, and reduced the swimming motility of These results indicate that GEON has the potential to inhibit the planktonic growth and biofilm formation of .

中文翻译：

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水纳米乳中大蒜精油对单核细胞增生李斯特氏菌的抗菌、抗生物膜活性及其潜在机制

据报道，水纳米乳（GEON）中的大蒜精油（GEO）具有良好的稳定性和广谱抗菌活性，但其作用却很少研究。在本研究中，研究了 GEON 的抗菌、抗生物膜形成活性及其潜在机制。结果表明，GEON（平均粒径=136.43 nm）显着抑制浮游生物的生长，纳米乳中GEO的最低抑制浓度（MIC）为512 μg/mL。GEON 可以以时间和浓度依赖的方式有效地杀死细菌。GEON处理后，细胞膜严重受损，膜明显去极化，细胞内物质（核酸、蛋白质和ATP）大量损失。此外，膜完整性的降低和细胞形态的塌陷进一步证实了细胞膜的损伤。此外，GEON 显着减弱了生物膜的形成，增强了表面疏水性，并降低了浮游生物的游动性。这些结果表明，GEON 具有抑制浮游生长和生物膜形成的潜力。