Small heat shock proteins (sHsps) promote microbial resistance to harsh environmental conditions. Three sHsp genes () from and one () from were cloned and transformed into MG1363, which does not contain sHSPs for heterologous expression. Comparing the tolerance of different recombinant strains to ethanol, the . gene with the highest tolerance was selected and inserted into the expression vector pHH40 and transformed into the new host . . The results showed that when compared with the ethanol tolerance of the original . strain, i.e., 12% , the recombinant strain could tolerate approximately 14% . Moreover, the recombinant strain shortened the malo-lactic fermentation time of wine by 2 days without any adverse effects on aroma. Therefore, the recombinant . strain showed better application prospects.

中文翻译：

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酒酒球菌异源表达小热休克蛋白可提高其乙醇耐受性

小热休克蛋白 (sHsps) 促进微生物对恶劣环境条件的抵抗力。克隆了 3 个 sHsp 基因和 1 个 sHsp 基因，并将其转化到 MG1363 中，该基因不含用于异源表达的 sHSP。比较不同重组菌株对乙醇的耐受性。选择耐受性最高的基因插入表达载体pHH40并转化新宿主。。结果表明，与原来的乙醇耐受性相比。菌株，即12%，重组菌株可以耐受约14%。而且，重组菌株使葡萄酒的苹果酸-乳酸发酵时间缩短了2天，且对香气没有任何不利影响。因此，重组 . 菌株显示出较好的应用前景。