A voltammetric sensor comprising a paste electrode made of graphitized thermal carbon black CarboblackC and nanosized zeolite MFI was developed for selective recognition and determination of tryptophan enantiomers. It was found that in determining tryptophan enantiomers, the dependence remains linear at concentrations from 5×10⁻⁵ to 1×10⁻³ M. The detection limits of tryptophan L- and D-enantiomers were calculated to be 4.98×10⁻⁷ M and 3.20×10⁻⁷ M, while their lower limits of determinable concentrations–1.66×10⁻⁶ M and 1.07×10⁻⁶ M, respectively. The sensor is more sensitive to D-tryptophan (ΔE_p=20 mV, i_pD/i_pL=1.58). The developed sensor was shown to successfully determine tryptophan enantiomers in model solutions of pure enantiomers, in a mixture of enantiomers, in biological fluids, and in tablet forms of biologically active additives. It was shown that the suggested sensor made it possible to determine the ratio of L- and D-enantiomers of tryptophan in a mixture with a high probability.

中文翻译：

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基于纳米MFI沸石的手性伏安传感器用于识别和测定色氨酸对映体

开发了一种伏安传感器，包括由石墨化热炭黑 CarboblackC 和纳米沸石 MFI 制成的糊状电极，用于选择性识别和测定色氨酸对映体。结果发现，在测定色氨酸对映体时，在5×10 ^-5至1×10 ^-3  M浓度范围内，依赖性保持线性。色氨酸L-和D-对映体的检测限经计算为4.98×10 ^-7  M和3.20×10 ^-7  M，而它们的可测定浓度下限分别为1.66×10 ^-6  M和1.07×10 ^-6  M。该传感器对 D-色氨酸更敏感（Δ E _p =20 mV，i _pD / i _pL =1.58）。所开发的传感器被证明可以成功测定纯对映体模型溶液、对映体混合物、生物液体以及生物活性添加剂片剂形式中的色氨酸对映体。结果表明，所提出的传感器可以以高概率确定混合物中色氨酸的 L-和 D-对映体的比例。