The use of glucose oxidase (GOx) to disrupt glucose supply has been identified as a promising strategy in cancer starvation therapy. However, independent delivery of GOx is prone to degradation upon exposure to biological conditions and may cause damage to blood vessels and normal organs during transportation. Although some carriers can protect GOx from the surrounding environment, the harsh preparation conditions may compromise its activity. Moreover, the commonly used materials often exhibit poor biocompatibility and possess certain cytotoxicity. To address this issue, we developed a gentle and efficient method based on Pickering emulsion templates to synthesize protein-based microparticles using zein as the matrix material. These microparticles have high stability and can be tailored to efficiently encapsulate biomolecules while preserving their activity. Moreover, the zein-based microparticles can be triggered to release biomolecules in tumor cells under high glutathione levels, demonstrating excellent responsiveness, biocompatibility, and low cytotoxicity. Additionally, when loaded with GOx, these protein-based microparticles effectively deprive tumor cells of nutrients and induce apoptosis by generating high levels of H₂O₂, thereby exhibiting enhanced anticancer properties.

中文翻译：

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皮克林乳液模板蛋白质微粒作为肿瘤细胞内吞作用的谷胱甘肽响应载体

使用葡萄糖氧化酶（GOx）来破坏葡萄糖供应已被认为是癌症饥饿治疗中一种有前景的策略。然而，独立递送的GOx在暴露于生物条件时容易降解，并且可能在运输过程中对血管和正常器官造成损害。尽管一些载体可以保护 GOx 免受周围环境的影响，但恶劣的制备条件可能会损害其活性。此外，常用材料往往生物相容性较差，并具有一定的细胞毒性。为了解决这个问题，我们开发了一种基于皮克林乳液模板的温和有效的方法，以玉米蛋白作为基质材料合成基于蛋白质的微粒。这些微粒具有高稳定性，可以定制以有效封装生物分子，同时保留其活性。此外，在高谷胱甘肽水平下，基于玉米醇溶蛋白的微粒可以在肿瘤细胞中被触发释放生物分子，表现出优异的响应性、生物相容性和低细胞毒性。此外，当负载GOx时，这些基于蛋白质的微粒有效地剥夺肿瘤细胞的营养并通过产生高水平的H ₂ O ₂诱导细胞凋亡，从而表现出增强的抗癌特性。