Nonribosomal peptide synthetases produce diverse natural products, including many valuable therapeutics. Although the condensation domains that catalyze peptide bond formation in these multifunctional enzymes have been difficult to engineer, a yeast display system that was developed to screen millions of variants now enables efficient reprogramming of synthetase substrate specificity.

中文翻译：

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生物合成装配线的高通量工程

非核糖体肽合成酶产生多种天然产物，包括许多有价值的治疗药物。尽管在这些多功能酶中催化肽键形成的缩合结构域很难设计，但开发用于筛选数百万个变体的酵母展示系统现在能够有效地重新编程合成酶底物特异性。