Allergen-specific immunoglobulin E (IgE) antibodies mediate pathology in diseases such as allergic rhinitis and food allergy. Memory B cells (MBCs) contribute to circulating IgE by regenerating IgE-producing plasma cells upon allergen encounter. Here, we report a population of type 2–polarized MBCs defined as CD23 hi , IL-4Rα hi , and CD32 low at both the transcriptional and surface protein levels. These MBC2s are enriched in IgG1- and IgG4-expressing cells while constitutively expressing germline transcripts for IgE. Allergen-specific B cells from patients with allergic rhinitis and food allergy were enriched in MBC2s. Furthermore, MBC2s generated allergen-specific IgE during sublingual immunotherapy, thereby identifying these cells as a major reservoir for IgE. The identification of MBC2s provides insights into the maintenance of IgE memory, which is detrimental in allergic diseases but could be beneficial in protection against venoms and helminths.

中文翻译：

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2 型极化记忆 B 细胞保存过敏原特异性 IgE 记忆

过敏原特异性免疫球蛋白 E (IgE) 抗体介导过敏性鼻炎和食物过敏等疾病的病理学。记忆 B 细胞 (MBC) 在遇到过敏原时通过再生产生 IgE 的浆细胞来促进 IgE 循环。在这里，我们报告了定义为 CD23 的 2 型极化 MBC 群体你好, IL-4Rα你好、CD32低的在转录和表面蛋白水平上。这些 MBC2 在表达 IgG1 和 IgG4 的细胞中富集，同时组成型表达 IgE 种系转录物。来自过敏性鼻炎和食物过敏患者的过敏原特异性 B 细胞在 MBC2 中富集。此外，MBC2 在舌下免疫治疗期间产生过敏原特异性 IgE，从而将这些细胞识别为 IgE 的主要储存库。 MBC2 的鉴定为 IgE 记忆的维持提供了见解，IgE 记忆对过敏性疾病有害，但可能有益于预防毒液和蠕虫。