The pathological expansion of white adipose tissue (WAT) in obesity involves adipocyte hypertrophy accompanied by expansion of the collagen-rich pericellular extracellular matrix (ECM) and development of crown-like structures (CLS). Traditionally, WAT morphology is assessed through immunohistochemical analysis of WAT sections. However, manual analysis of large histological sections is time-consuming, and the available digital tools for analyzing adipocyte size and pericellular ECM are limited. To address this gap, the authors developed the Adipose Tissue Analysis Toolkit (ATAT), an ImageJ plugin facilitating analysis of adipocyte size, WAT ECM, and CLS.

中文翻译：

---

脂肪组织分析工具包 (ATAT)，用于自动分析白色脂肪组织中的脂肪细胞大小和细胞外基质

肥胖症中白色脂肪组织（WAT）的病理性扩张涉及脂肪细胞肥大，伴随着富含胶原蛋白的细胞周细胞外基质（ECM）的扩张和冠状结构（CLS）的发育。传统上，WAT 形态是通过 WAT 切片的免疫组织化学分析来评估的。然而，大型组织切片的手动分析非常耗时，并且用于分析脂肪细胞大小和细胞周 ECM 的可用数字工具有限。为了解决这一差距，作者开发了脂肪组织分析工具包 (ATAT)，这是一个 ImageJ 插件，有助于分析脂肪细胞大小、WAT ECM 和 CLS。