In this research, a novel polysaccharide-based complex system was developed through self-assembly driven by electrostatic interactions between carboxymethyl (CM)-modified tamarind seed polysaccharide (TSP) and chitosan (CS). First, the carboxymethylation of TSP was confirmed successfully. Then, the relationships among the morphology, physicochemical properties and interfacial activity of CMTSP/CS complexes with different mass ratios (1:5, 1:3, 1:1, 3:1 and 5:1) were systematically investigated. The turbidity of the complex solution gradually increased with higher mass ratios (CMTSP:CS) and pH values. The size of the complex initially decreased and then increased as the proportion of CMTSP increased. The CMTSP/CS complex at 1:1 displayed the smallest particle size, supreme surface wettability and strongest interfacial activity. The microstructure and rheological properties of emulsions stabilized by CMTSP/CS complexes were characterized. CMTSP/CS complexes at 1:1 and 1:3 exhibited enhanced emulsifying capacity and better viscoelastic attributes. Remarkably, stable high internal phase emulsions with 85% oil concentration could be formed using very low concentration (only 0.05 wt%). These findings suggest that CMTSP/CS complex-stabilized emulsions have great application potential as a new oil-in-water system.

中文翻译：

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羧甲基罗望子种子多糖/壳聚糖复合物通过静电相互作用稳定高内相乳液：质量比和油水界面活性的作用

在这项研究中，通过羧甲基（CM）修饰的罗望子种子多糖（TSP）和壳聚糖（CS）之间的静电相互作用驱动的自组装，开发了一种新型的基于多糖的复合系统。首先，成功证实了TSP的羧甲基化。然后系统研究了不同质量比（1:5、1:3、1:1、3:1和5:1）的CMTSP/CS复合物的形貌、理化性质和界面活性之间的关系。络合物溶液的浊度随着质量比（CMTSP:CS）和pH值的升高而逐渐增加。随着 CMTSP 比例的增加，复合物的尺寸先减小后增大。 1:1 的 CMTSP/CS 复合物表现出最小的粒径、最高的表面润湿性和最强的界面活性。对 CMTSP/CS 复合物稳定的乳液的微观结构和流变性能进行了表征。 1:1 和 1:3 的 CMTSP/CS 复合物表现出增强的乳化能力和更好的粘弹性属性。值得注意的是，使用非常低的浓度（仅 0.05 wt%）就可以形成油浓度为 85% 的稳定的高内相乳液。这些研究结果表明，CMTSP/CS复合稳定乳液作为新型水包油体系具有巨大的应用潜力。