Recent advances in single-cell and multicellular microfluidics technology have provided powerful tools for studying cancer biology and immunology. The ability to create controlled microenvironments, perform high-throughput screenings, and monitor cellular interactions at the single-cell level has significantly advanced our understanding of tumor biology and immune responses. We discuss cutting-edge multicellular and single-cell microfluidic technologies and methodologies utilized to investigate cancer–immune cell interactions and assess the effectiveness of immunotherapies. We explore the advantages and limitations in the wide range of 3D spheroid and single-cell microfluidic models recently developed, highlighting the various approaches in device generation and applications in immunotherapy screening for potential opportunities for point-of-care approaches.Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 26 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

中文翻译：

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利用液滴微流控推进即时护理应用：从单细胞到多细胞分析

单细胞和多细胞微流体技术的最新进展为研究癌症生物学和免疫学提供了强大的工具。创建受控微环境、进行高通量筛选以及在单细胞水平上监测细胞相互作用的能力极大地增进了我们对肿瘤生物学和免疫反应的理解。我们讨论用于研究癌症-免疫细胞相互作用并评估免疫疗法有效性的尖端多细胞和单细胞微流体技术和方法。我们探讨了最近开发的各种 3D 球体和单细胞微流体模型的优点和局限性，重点介绍了设备生成的各种方法以及免疫治疗筛选中的应用，以寻找床旁护理方法的潜在机会。预计最终在线发布日期《生物医学工程年度评论》第 26 卷的发布时间为 2024 年 5 月。请参阅 http://www.annualreviews.org/page/journal/pubdates 了解修订后的估计。