Rapeseed ( L.) is one of the most important oil crops worldwide. However, its yield is greatly limited by salt stress, one of the primary abiotic stresses. Identification of salt-tolerance genes and breeding salt-tolerant varieties is an effective approach to address this issue. Unfortunately, little is known about the salt-tolerance quantitative trait locus (QTL) and the identification of salt tolerance genes in rapeseed. In this study, high-throughput quantitative trait locus sequencing (QTL-seq) was applied to identifying salt-tolerant major QTLs based on two DNA pools from an F population of a cross between rapeseed line 2205 (salt tolerant) and 1423 (salt sensitive). A total of twelve major QTLs related to the salt tolerance rating (STR) were detected on chromosomes A03, A08, C02, C03, C04, C06, C07 and C09. To further enhance our understanding, we integrated QTL-seq data with transcriptome analysis of the two parental rapeseed plants subjected to salt stress, through which ten candidate genes for salt tolerance were identified within the major QTLs by gene differential expression, variation and annotated functions analysis. The marker SNP820 linked to salt tolerance was successfully validated and would be useful as a diagnostic marker in marker-assisted breeding. These findings provide valuable insights for future breeding programs aimed at developing rapeseed cultivars resistant to salt stresses.

中文翻译：

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通过甘蓝型油菜数量性状基因座测序鉴定抗盐性候选基因。


油菜籽（L.）是世界上最重要的油料作物之一。然而，其产量受到盐胁迫（主要非生物胁迫之一）的极大限制。鉴定耐盐基因并选育耐盐品种是解决这一问题的有效途径。不幸的是，人们对油菜籽耐盐数量性状基因座（QTL）和耐盐基因的鉴定知之甚少。在这项研究中，应用高通量数量性状基因座测序（QTL-seq）来鉴定耐盐主要QTL，该QTL基于来自油菜品系2205（耐盐）和1423（盐敏感）杂交的F群体的两个DNA库。 ）。在A03、A08、C02、C03、C04、C06、C07和C09染色体上共检测到12个与耐盐等级（STR）相关的主要QTL。为了进一步加深我们的理解，我们将QTL-seq数据与遭受盐胁迫的两个亲本油菜植株的转录组分析相结合，通过基因差异表达、变异和注释功能分析，在主要QTL中鉴定出10个耐盐候选基因。 。与耐盐性相关的标记 SNP820 已成功验证，可用作标记辅助育种中的诊断标记。这些发现为未来旨在开发抗盐胁迫的油菜品种的育种计划提供了宝贵的见解。