The effects of xylo-oligosaccharides (XOS) on broiler growth performance, immune function, and intestinal health were investigated. A total of 540 one-day-old Arbor Acres Plus broilers were randomly divided into 5 groups with 6 replicates per group and 18 chickens per replicate. Broilers in the control (CON) group received a corn-soybean meal based basal diet, those in the antibiotics (ANT) group received the basal diet plus 500 mg/kg oxytetracycline, and those in XOS groups received the basal diet plus 150, 300, and 450 mg/kg XOS, respectively. Compared with CON, the body weight at 42 d and average daily gain from 1 to 42 d were significantly increased in the 150, 450 mg/kg XOS-added and ANT groups ( = 0.018), and the relative expression of claudin-1 and mRNA in the ileum was significantly higher in the 300 and 450 mg/kg XOS-added groups ( < 0.001). The feed conversion ratios ( < 0.001) and abdominal fat rates (possibly related to short-chain fatty acids [butyric acid] produced by XOS in broilers) ( = 0.012) of broilers from 1 to 42 d of age were significantly lower in all XOS-added groups than in the control group. Splenic index ( = 0.036) and bursa index ( = 0.009) were significantly better in the ANT group and each XOS-added group than in the control group. Compared to CON and ANT, serum IgA ( = 0.007) and IgG ( = 0.002) levels were significantly higher in the 300 mg/kg XOS-added group, and the relative abundance of short-chain fatty acid-producing genera () was also significantly higher ( < 0.001). Meanwhile, ileal villus height ( < 0.001) and V:C ( = 0.001) were significantly increased in XOS-added broilers. In analysis of relationships between cecal microbes and the physical barrier of the gut, was positively correlated with mRNA expression of ileal claudin-1 ( < 0.05), and was positively correlated with increased ileal villus height and V:C ( < 0.05). Overall, XOS addition to broiler diets improved growth performance, promoted intestinal health by enhancing intestinal barrier function, and regulating cecal microbiota diversity, and had positive effects on immunity.

中文翻译：

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日粮中添加低聚木糖可改善肉鸡的生长性能、免疫功能和肠道健康

研究了低聚木糖 (XOS) 对肉鸡生长性能、免疫功能和肠道健康的影响。将 540 只 1 日龄 Arbor Acres Plus 肉鸡随机分为 5 组，每组 6 个重复，每个重复 18 只鸡。对照组（CON）组的肉鸡接受以玉米-豆粕为主的基础日粮，抗生素（ANT）组的肉鸡接受基础日粮加 500 mg/kg 土霉素，XOS 组的肉鸡接受基础日粮加 150、300 mg/kg 土霉素。 、 和 450 mg/kg XOS，分别。与 CON 相比，添加 150、450 mg/kg XOS 和 ANT 组的 42 d 体重和 1~42 d 平均日增重均显着增加（=0.018），claudin-1 和添加 300 毫克/千克和 450 毫克/千克低聚木糖的组中，回肠中的 mRNA 显着较高（< 0.001）。所有 XOS 中 1 至 42 日龄肉鸡的饲料转化率 ( < 0.001) 和腹脂率 (可能与肉鸡中 XOS 产生的短链脂肪酸[丁酸]有关) ( = 0.012) 显着较低-添加的组数高于对照组。ANT组和各添加低聚木糖组的脾指数(=0.036)和法氏囊指数(=0.009)显着优于对照组。与 CON 和 ANT 相比，添加 300 mg/kg XOS 的组血清 IgA ( = 0.007) 和 IgG ( = 0.002) 水平显着升高，并且产生短链脂肪酸属的相对丰度也显着升高。显着更高（<0.001）。同时，添加 XOS 的肉鸡的回肠绒毛高度 (< 0.001) 和 V:C (= 0.001) 显着增加。在盲肠微生物与肠道物理屏障之间的关系分析中，与回肠claudin-1的mRNA表达呈正相关（<0.05），与回肠绒毛高度和V:C的增加呈正相关（<0.05）。总体而言，肉鸡日粮中添加低聚木糖可改善生长性能，通过增强肠道屏障功能和调节盲肠微生物群多样性来促进肠道健康，并对免疫力产生积极影响。