Background

Spinal cord injury (SCI) remained one of the challenges to treat due to its complicated mechanisms. Photobiomodulation therapy (PBMT) accelerates neuronal regeneration. Cerium oxide nanoparticles (CeONPs) also eliminate free radicals in the environment. The present study aims to introduce a combined treatment method of making PCL scaffolds as microenvironments, seeded with CeONPs and the PBMT technique for SCI treatment.

Methods

The surgical hemi-section was used to induce SCI. Immediately after the SCI induction, the scaffold (Sc) was loaded with CeONPs implanted. PBMT began 30 min after SCI induction and lasted for up to 4 weeks. Fifty-six male rats were randomly divided into seven groups. Glial fibrillary acidic protein (GFAP) (an astrocyte marker), Connexin 43 (Con43) (a member of the gap junction), and gap junctions (GJ) (a marker for the transfer of ions and small molecules) expressions were evaluated. The behavioral evaluation was performed by BBB, Acetone, Von Frey, and radiant heat tests.

Result

The SC + Nano + PBMT group exhibited the most remarkable recovery outcomes. Thermal hyperalgesia responses were mitigated, with the combined approach displaying the most effective relief. Mechanical allodynia and cold allodynia responses were also attenuated by treatments, demonstrating potential pain management benefits.

Conclusion

These findings highlight the potential of PBMT, combined with CeONPs-loaded scaffolds, in promoting functional motor recovery and alleviating pain-related responses following SCI. The study underscores the intricate interplay between various interventions and their cumulative effects, informing future research directions for enhancing neural repair and pain management strategies in SCI contexts.

中文翻译：

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负载氧化铈纳米粒子的支架和光生物调节疗法对脊髓损伤后疼痛和神经元再生的联合作用：实验研究

背景

由于其复杂的机制，脊髓损伤（SCI）仍然是治疗的挑战之一。光生物调节疗法（PBMT）可加速神经元再生。氧化铈纳米颗粒 (CeONP) 还可消除环境中的自由基。本研究旨在介绍一种以 PCL 支架为微环境、接种 CeONPs 和 PBMT 技术用于 SCI 治疗的组合治疗方法。

方法

手术半切片用于诱导 SCI。 SCI 诱导后，支架 (Sc) 上立即装载植入的 CeONP。 PBMT 在 SCI 诱导后 30 分钟开始，持续长达 4 周。 56 只雄性大鼠随机分为七组。评估了胶质纤维酸性蛋白 (GFAP)（星形胶质细胞标记物）、连接蛋白 43 (Con43)（间隙连接的成员）和间隙连接 (GJ)（离子和小分子转移的标记物）的表达。通过 BBB、丙酮、Von Frey 和辐射热测试进行行为评估。

结果

SC + Nano + PBMT 组表现出最显着的恢复效果。热痛觉过敏反应得到缓解，联合方法显示出最有效的缓解效果。机械异常性疼痛和冷异常性疼痛反应也通过治疗减弱，这表明潜在的疼痛管理益处。

结论

这些发现凸显了 PBMT 与负载 CeONP 的支架相结合在促进功能性运动恢复和减轻 SCI 后疼痛相关反应方面的潜力。该研究强调了各种干预措施及其累积效应之间错综复杂的相互作用，为未来加强 SCI 背景下的神经修复和疼痛管理策略的研究方向提供了信息。