当前位置: X-MOL 学术Environ. Exp. Bot. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Transcriptome analysis reveals salt stress-related genes in Rhododendron simii and RsWRKY40 is referred to salt tolerance
Environmental and Experimental Botany ( IF 4.5 ) Pub Date : 2024-02-01 , DOI: 10.1016/j.envexpbot.2024.105678
Liping Guo , Xia Mao , You Chen , Li Li , Yanrong Hu , Hanghang Zhang , Rui Zhang , Yunkun Wang

The plants of genus hold significant economic and ornamental value. Investigating their response to salt stress is critical to breeding salt-tolerant cultivars, but this area has been relatively limited. This study utilized transcriptome analysis to identify salt stress-related genes in . Through DEGs, GO and KEGG enrichment, and expression pattern analysis, 18 candidates involved in intrinsic component of membrane, oxidoreductase activity, amino sugar and nucleotide sugar metabolism, and fructose and mannose metabolism, were identified. Additionally, through weighted gene co-expression network analysis (WGCNA), 10 candidates related to arginine and proline metabolism, plant hormone signal transduction, and amino sugar and nucleotide sugar metabolism were further screened out. Transcription factor (TF) analysis showed that 35 TFs responded to salt stress in . The overexpression of in Arabidopsis increased seed germination rate, root length, and seedling survival rate under salt conditions, suggesting potential role in enhancing salt tolerance in transgenic Arabidopsis, making it a promising candidate for improving salt resistance in . These findings will provide valuable theoretical and genetic resources for breeding salt-tolerant cultivars.

中文翻译:

转录组分析揭示杜鹃中盐胁迫相关基因,RsWRKY40被称为耐盐性

该属植物具有重要的经济和观赏价值。研究它们对盐胁迫的反应对于培育耐盐品种至关重要,但这一领域相对有限。本研究利用转录组分析来鉴定 中与盐胁迫相关的基因。通过DEG、GO和KEGG富集以及表达模式分析,鉴定出涉及膜内在成分、氧化还原酶活性、氨基糖和核苷酸糖代谢、果糖和甘露糖代谢的18个候选蛋白。此外,通过加权基因共表达网络分析(WGCNA),进一步筛选出与精氨酸和脯氨酸代谢、植物激素信号转导、氨基糖和核苷酸糖代谢相关的10个候选基因。转录因子 (TF) 分析表明 35 个 TF 对盐胁迫有反应。在拟南芥中过表达增加了盐条件下种子的发芽率、根长和幼苗存活率,表明其在增强转基因拟南芥耐盐性方面具有潜在作用,使其成为提高拟南芥耐盐性的有希望的候选者。这些发现将为选育耐盐品种提供宝贵的理论和遗传资源。
更新日期:2024-02-01
down
wechat
bug