Leaf senescence represents an active and regulated degeneration process. Leucine-rich repeat receptor-like protein kinase (LRR-RLK) represents a large group of cell surface receptors that play important roles in multiple biological processes, and some are also known to regulate the senescence process. In the present study, we elucidated the involvement of Phytosulfokine Receptor (PSKR) gene in the regulation of leaf senescence. The expression of of Arabidopsis was found to be up-regulated during leaf senescence. The over-expressing lines of and its rice homologue displayed an early leaf senescent phenotype in Arabidopsis, while the late senescence in mutant line of supported the idea that positively regulate the process of leaf senescence. Furthermore, the leaves of the overexpressing lines displayed increased superoxide accumulation compared to mutant lines, along with higher expression of NADPH oxidases ( and ). Importantly, our findings demonstrates that both AtPSKR1and OsPSKR10 interact with AtRBOHD and AtRBOHF and thereby instigating superoxide accumulation in the leaves through NADPH oxidases.

中文翻译：

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拟南芥中植物硫因子受体 1 (AtPSKR1) 通过介导 ROS 信号传导作为叶片衰老的正调节因子

叶子衰老代表一个活跃且受调节的退化过程。富含亮氨酸重复受体样蛋白激酶（LRR-RLK）代表一大类细胞表面受体，在多种生物过程中发挥重要作用，其中一些受体还可以调节衰老过程。在本研究中，我们阐明了植物硫因子受体（PSKR）基因在叶片衰老调控中的作用。发现拟南芥的表达在叶片衰老过程中上调。超表达品系及其水稻同源物在拟南芥中表现出早期叶片衰老表型，而突变品系中的晚衰老支持了正调控叶片衰老过程的观点。此外，与突变株系相比，过表达株系的叶子表现出超氧化物积累增加，并且 NADPH 氧化酶表达更高（和）。重要的是，我们的研究结果表明 AtPSKR1 和 OsPSKR10 都与 AtRBOHD 和 AtRBOHF 相互作用，从而通过 NADPH 氧化酶促进叶片中超氧化物的积累。