Fufang Xiling Jiedu capsule (FXJC), a traditional Chinese medicine that evolved from “Yinqiao Powder”, is widely used for the treatment of cold and influenza. However, due to a lack of in vivo metabolism research, the chemical components responsible for the therapeutic effects still remain unclear. Hence, this study aimed to describe the metabolic profiles of the FXJC in rat plasma, urine, and feces. A combined data mining strategy based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was employed and 201 xenobiotics, including 117 prototype components and 84 metabolites were detected. Phenolic acids, flavonoids, triterpenes, and lignans were prominent ingredients absorbed in vivo, and the major metabolic pathways of the detected metabolites were glucuronidation, sulfation, methylation, and oxidation. This is the first systematic study on the metabolism of the FXJC in vivo, providing valuable information for future studies on the efficacy, toxicity, and mechanism of the FXJC.

中文翻译：

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超高效液相色谱-四极杆飞行时间串联质谱法研究复方西灵解毒胶囊在大鼠体内的代谢特征

复方西灵解毒胶囊（FXJC）是由“银翘散”演变而来的中药，广泛用于治疗感冒和流感。然而，由于缺乏体内代谢研究，导致治疗效果的化学成分仍不清楚。因此，本研究旨在描述大鼠血浆、尿液和粪便中 FXJC 的代谢特征。采用基于超高效液相色谱与四极杆飞行时间串联质谱联用的组合数据挖掘策略，检测到 201 种外源物质，包括 117 种原型成分和 84 种代谢物。酚酸、黄酮类、三萜类和木脂素是体内吸收的主要成分，检测到的代谢物的主要代谢途径是葡萄糖醛酸化、硫酸化、甲基化和氧化。这是首次对FXJC体内代谢进行系统研究，为今后研究FXJC的功效、毒性和作用机制提供了有价值的信息。