This study aims to prepare Z-isomer-rich lycopene nanodispersions via ethanol extraction at higher temperature and pressure conditions (i.e., subcritical conditions) from tomato powder and continuous dispersion using an in-line swirl mixer. Firstly, the effects of ethanol extraction conditions, i.e., temperature (40–160 ℃), time (10–60 min), and pressure (10–30 MPa), on lycopene recovery and Z-isomerization were investigated. The extraction at > 120 ℃ significantly enhanced lycopene recovery and Z-isomer ratio of lycopene in the resulting extract. Subsequently, the resulting extract liquid (ethanol solution) was continuously dispersed in water containing polyglycerol fatty acid ester using an in-line mixer, and successfully prepared Z-isomer-rich (approximately 70%) lycopene nanodispersions. This production process is economical and highly compatible with the food industry due to using a safe extraction solvent and continuous production capability. In the future, it will be necessary to investigate the biological activities of the resulting lycopene and perform scale-up verification of this process.

本研究旨在通过在较高温度和压力条件（即亚临界条件）下从番茄粉中乙醇提取并使用在线旋流混合器连续分散来制备富含Z异构体的番茄红素纳米分散体。首先，考察了乙醇提取条件，即温度（40~160 ℃）、时间（10~60  min）和压力（10~30  MPa）对番茄红素回收率和Z异构化的影响。> 120 ℃的提取显着提高了所得提取物中番茄红素的回收率和番茄红素的Z异构体比例。随后，使用在线混合器将所得提取液（乙醇溶液）连续分散在含有聚甘油脂肪酸酯的水中，并成功制备富含Z异构体（约70%）的番茄红素纳米分散体。由于使用安全的提取溶剂和连续生产能力，该生产工艺经济且与食品工业高度兼容。未来，有必要研究所得番茄红素的生物活性，并对该过程进行放大验证。