In this study, the effects of phosphate buffered saline (PBS) and graphene oxide (GO) as supporting electrolytes and dopants on the electropolymerization process of 3,4-ethylenedioxythiophene (EDOT) on glassy carbon electrode (GCE) were investigated. It was found that the PEDOT-ERGO nanocomposites obtained by a simple one-step electrochemical redox polymerization method using GO as the only supporting electrolyte and dopant possess excellent electrochemical properties. Then, the PEDOT-ERGO nanocomposites were used as electrode substrate to further modify with AuNPs, and an electrochemical aptasensor based on AuNPs/PEDOT-ERGO nanocomposites was successfully constructed for the sensitive and selective determination of dopamine (DA). Comparison of the cyclic voltammetric response of different neurotransmitters before and after aptamer assembly showed that the aptamer significantly improved the selectivity of the sensor for DA. The low detection limit of 1.0 μM (S/N = 3) indicated the good electrochemical performance of the PEDOT-ERGO nanocomposite film. Moreover, the aptasensor showed good recoveries in 50-fold diluted fetal bovine serum with RSD values all less than 5 % (n = 5), indicating that the PEDOT-ERGO nanocomposites and the electrochemical aptasensor have promising applications in other neurochemicals assay and biomedical analysis.

中文翻译：

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基于 AuNPs/PEDOT-ERGO 纳米复合材料的灵敏选择性电化学适体传感方法用于伏安法测定多巴胺

本研究研究了磷酸盐缓冲盐水（PBS）和氧化石墨烯（GO）作为支持电解质和掺杂剂对3,4-乙撑二氧噻吩（EDOT）在玻碳电极（GCE）上电聚合过程的影响。研究发现，以GO作为唯一的支持电解质和掺杂剂，通过简单的一步电化学氧化还原聚合方法获得的PEDOT-ERGO纳米复合材料具有优异的电化学性能。然后，以PEDOT-ERGO纳米复合材料为电极基底，对AuNPs进行进一步修饰，成功构建了基于AuNPs/PEDOT-ERGO纳米复合材料的电化学适体传感器，用于灵敏、选择性测定多巴胺（DA）。适配体组装前后不同神经递质循环伏安响应的比较表明，适配体显着提高了传感器对DA的选择性。 1.0 μM（S/N = 3）的低检测限表明PEDOT-ERGO纳米复合薄膜具有良好的电化学性能。此外，适配体传感器在50倍稀释的胎牛血清中表现出良好的回收率，RSD值均小于5%（n = 5），表明PEDOT-ERGO纳米复合材料和电化学适配体传感器在其他神经化学分析和生物医学分析中具有广阔的应用前景。