Drosophila geneticists frequently employ the binary GAL4-UAS system of conditional gene expression to direct expression of the desired transgene in tissues of interest. The inscuteable -GAL4 driver (insc-GAL4) expresses in the type 1 and type 2 neuroblasts of Drosophila larval brain, a frequent target tissue in many investigations. This GAL4 line additionally displayed its expression in the midgut. In this study, we examined the expression of the UAS-mCD8GFP reporter under the command of the insc-GAL4 driver and observed that this driver expresses exclusively to intestinal stem cells (ISCs) of the Drosophila adult midgut as well as adult midgut precursors (AMPs) of the larval midgut besides its expression in larval brain. Additionally, using the G-TRACE method, it was observed that AMPs in the larval midgut consistently expressed insc-GAL4 in real-time, and the lineage expression of this GAL4 was observed in the enterocyte cells. This study reveals for the first time that insc-GAL4 is specific to larval AMPs and adult ISCs of the midgut.

果蝇遗传学家经常使用条件基因表达的二元GAL4-UAS系统来指导所需转基因在感兴趣的组织中的表达。inscuteable -GAL4驱动程序( insc-GAL4 ) 在果蝇幼虫脑的 1 型和 2 型神经母细胞中表达，果蝇幼虫脑是许多研究中常见的靶组织。该GAL4系还在中肠中表达。在这项研究中，我们检查了insc-GAL4驱动程序指挥下的UAS-mCD8GFP报告基因的表达，并观察到该驱动程序仅表达于果蝇成体中肠的肠干细胞 (ISC)以及成体中肠前体细胞 (AMP) ）除了在幼虫脑中表达外，还包括幼虫中肠的表达。此外，使用G-TRAC E方法，观察到幼虫中肠中的AMPs实时一致表达insc-GAL4 ，并且在肠上皮细胞中观察到该GAL4的谱系表达。这项研究首次揭示了insc-GAL4对中肠幼虫 AMP 和成虫 ISC 具有特异性。