The present research aims to evaluate the efficacy of Silibinin-loaded mesoporous silica nanoparticles (Sil@MSNs) immobilized into polylactic-co-glycolic acid/Collagen (PLGA/Col) nanofibers on the in vitro proliferation of adipose-derived stem cells (ASCs) and cellular senescence. Here, the fabricated electrospun PLGA/Col composite scaffolds were coated with Sil@MSNs and their physicochemical properties were examined by FTIR, FE-SEM, and TGA. The growth, viability and proliferation of ASCs were investigated using various biological assays including PicoGreen, MTT, and RT-PCR after 21 days. The proliferation and adhesion of ASCs were supported by the biological and mechanical characteristics of the Sil@MSNs PLGA/Col composite scaffolds, according to FE- SEM. PicoGreen and cytotoxicity analysis showed an increase in the rate of proliferation and metabolic activity of hADSCs after 14 and 21 days, confirming the initial and controlled release of Sil from nanofibers. Gene expression analysis further confirmed the increased expression of stemness markers as well as hTERT and telomerase in ASCs seeded on Sil@MSNs PLGA/Col nanofibers compared to the control group. Ultimately, the findings of the present study introduced Sil@MSNs PLGA/Col composite scaffolds as an efficient platform for long-term proliferation of ASCs in tissue engineering.

本研究旨在评估固定在聚乳酸-乙醇酸/胶原蛋白（PLGA/Col）纳米纤维中的水飞蓟宾介孔二氧化硅纳米颗粒（Sil@MSNs）对脂肪干细胞（ASC）体外增殖的功效和细胞衰老。在这里，制造的电纺 PLGA/Col 复合支架涂有 Sil@MSNs，并通过 FTIR、FE-SEM 和 TGA 检查其理化性质。 21 天后，使用各种生物测定法（包括 PicoGreen、MTT 和 RT-PCR）研究 ASC 的生长、活力和增殖。根据 FE-SEM，Sil@MSNs PLGA/Col 复合支架的生物和机械特性支持 ASC 的增殖和粘附。 PicoGreen 和细胞毒性分析显示，14 天和 21 天后，hADSC 的增殖率和代谢活性有所增加，证实了纳米纤维中 Sil 的初始和受控释放。基因表达分析进一步证实，与对照组相比，接种在 Sil@MSNs PLGA/Col 纳米纤维上的 ASC 中干性标记以及 hTERT 和端粒酶的表达增加。最终，本研究的结果介绍了 Sil@MSNs PLGA/Col 复合支架作为组织工程中 ASC 长期增殖的有效平台。