Endogenous electrophiles, ionizing and non-ionizing radiation, and hazardous chemicals present in the environment and diet can damage DNA by forming covalent adducts. DNA adducts can form in critical cancer driver genes and, if not repaired, may induce mutations during cell division, potentially leading to the onset of cancer. The detection and quantification of specific DNA adducts are some of the first steps in studying their role in carcinogenesis, the physiological conditions that lead to their production, and the risk assessment of exposure to specific genotoxic chemicals. Hundreds of different DNA adducts have been reported in the literature, and there is a critical need to establish a DNA adduct mass spectral database to facilitate the detection of previously observed DNA adducts and characterize newly discovered DNA adducts. We have collected synthetic DNA adduct standards from the research community, acquired MSⁿ (n = 2, 3) fragmentation spectra using Orbitrap and Quadrupole-Time-of-Flight (Q-TOF) MS instrumentation, processed the spectral data and incorporated it into the MassBank of North America (MoNA) database, and created a DNA adduct portal Web site (https://sites.google.com/umn.edu/dnaadductportal) to serve as a central location for the DNA adduct mass spectra and metadata, including the spectral database downloadable in different formats. This spectral library should prove to be a valuable resource for the DNA adductomics community, accelerating research and improving our understanding of the role of DNA adducts in disease.

中文翻译：

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DNA 加成组学质谱库

环境和饮食中存在的内源性亲电子试剂、电离和非电离辐射以及危险化学物质可能会通过形成共价加合物而损害 DNA。DNA 加合物可以在关键的癌症驱动基因中形成，如果不加以修复，可能会在细胞分裂过程中诱发突变，从而可能导致癌症的发生。特定 DNA 加合物的检测和定量是研究其在致癌作用、导致其产生的生理条件以及接触特定基因毒性化学物质的风险评估中的第一步。文献中已报道了数百种不同的DNA加合物，迫切需要建立DNA加合物质谱数据库，以方便检测先前观察到的DNA加合物并表征新发现的DNA加合物。我们从研究界收集了合成 DNA 加合物标准品，使用 Orbitrap 和四极杆飞行时间 (Q-TOF) MS 仪器获取了 MS ⁿ ( n = 2, 3) 碎片光谱，处理了光谱数据并将其纳入MassBank of North America (MoNA) 数据库，并创建了 DNA 加合物门户网站 (https://sites.google.com/umn.edu/dnaadductportal) 作为 DNA 加合物质谱和元数据的中心位置，包括可以不同格式下载的光谱数据库。该谱库应该被证明是 DNA 加合物组学界的宝贵资源，可加速研究并提高我们对 DNA 加合物在疾病中的作用的理解。