Ankylosing spondylitis (AS) is one of several disorders known as seronegative spinal arthritis (SpA), the origin of which is unknown. Existing epidemiological data show that inflammatory and immunological factors are important in the development of AS. Previous research on the connection between immunological inflammation and AS, however, has shown inconclusive results. To evaluate the causal association between immunological characteristics and AS, a bidirectional, two-sample Mendelian randomization (MR) approach was performed in this study. We investigated the causal connection between 731 immunological feature characteristic cells and AS risk using large, publically available genome-wide association studies. After FDR correction, two immunophenotypes were found to be significantly associated with AS risk: CD14 − CD16 + monocyte (OR, 0.669; 95% CI, 0.544 ~ 0.823; P = 1.46 × 10−4; PFDR = 0.043), CD33dim HLA DR + CD11b + (OR, 0.589; 95% CI = 0.446 ~ 0.780; P = 2.12 × 10−4; PFDR = 0.043). AS had statistically significant effects on six immune traits: CD8 on HLA DR + CD8 + T cell (OR, 1.029; 95% CI, 1.015 ~ 1.043; P = 4.46 × 10−5; PFDR = 0.014), IgD on IgD + CD24 + B cell (OR, 0.973; 95% CI, 0.960 ~ 0.987; P = 1.2 × 10−4; PFDR = 0.021), IgD on IgD + CD38 − unswitched memory B cell (OR, 0.962; 95% CI, 0.945 ~ 0.980; P = 3.02 × 10−5; PFDR = 0.014), CD8 + natural killer T %lymphocyte (OR, 0.973; 95% CI, 0.959 ~ 0.987; P = 1.92 × 10−4; PFDR = 0.021), CD8 + natural killer T %T cell (OR, 0.973; 95% CI, 0.959 ~ 0.987; P = 1.65 × 10−4; PFDR = 0.021). Our findings extend genetic research into the intimate link between immune cells and AS, which can help guide future clinical and basic research.

中文翻译：

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免疫细胞与强直性脊柱炎之间的因果关系：双向孟德尔随机化研究

强直性脊柱炎 (AS) 是血清阴性脊柱关节炎 (SpA) 的几种疾病之一，其起源尚不清楚。现有流行病学数据表明炎症和免疫因素在AS的发生发展中起重要作用。然而，先前关于免疫性炎症与 AS 之间关系的研究尚未得出结论。为了评估免疫学特征与 AS 之间的因果关系，本研究采用了双向、两个样本的孟德尔随机化 (MR) 方法。我们利用大型、公开的全基因组关联研究调查了 731 个免疫学特征细胞与 AS 风险之间的因果关系。FDR校正后，发现两种免疫表型与AS风险显着相关：CD14−CD16+单核细胞（OR，0.669；95% CI，0.544~0.823；P=1.46×10−4；PFDR=0.043）、CD33dim HLA DR + CD11b +（OR，0.589；95% CI = 0.446 ~ 0.780；P = 2.12 × 10−4；PFDR = 0.043）。AS 对 6 种免疫特征具有统计学显着影响：CD8 对 HLA DR + CD8 + T 细胞（OR，1.029；95% CI，1.015 ~ 1.043；P = 4.46 × 10−5；PFDR = 0.014），IgD 对 IgD + CD24 + B 细胞（OR，0.973；95% CI，0.960 ~ 0.987；P = 1.2 × 10−4；PFDR = 0.021），IgD 上的 IgD + CD38 − 未转换的记忆 B 细胞（OR，0.962；95% CI，0.945 ~ 0.980；P = 3.02 × 10−5；PFDR = 0.014），CD8 + 自然杀伤 T 淋巴细胞百分比（OR，0.973；95% CI，0.959 ~ 0.987；P = 1.92 × 10−4；PFDR = 0.021），CD8 +自然杀伤 T %T 细胞（OR，0.973；95% CI，0.959 ~ 0.987；P = 1.65 × 10−4；PFDR = 0.021）。我们的研究结果将基因研究扩展到免疫细胞与 AS 之间的密切联系，这有助于指导未来的临床和基础研究。