Circulating tumor DNA (ctDNA) has emerged as a promising tool for various clinical applications, including early diagnosis, therapeutic target identification, treatment response monitoring, prognosis evaluation, and minimal residual disease detection. Consequently, ctDNA assays have been incorporated into clinical practice. In this review, we offer an in-depth exploration of the clinical implementation of ctDNA assays. Notably, we examined existing evidence related to pre-analytical procedures, analytical components in current technologies, and result interpretation and reporting processes. The primary objective of this guidelines is to provide recommendations for the clinical utilization of ctDNA assays.

中文翻译：

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基于血液的循环肿瘤 DNA 测定的临床实践指南。

循环肿瘤 DNA (ctDNA) 已成为各种临床应用的有前景的工具，包括早期诊断、治疗靶点识别、治疗反应监测、预后评估和微小残留病检测。因此，ctDNA 检测已被纳入临床实践。在这篇综述中，我们对 ctDNA 检测的临床实施进行了深入探索。值得注意的是，我们检查了与分析前程序、当前技术中的分析组件以及结果解释和报告流程相关的现有证据。本指南的主要目的是为 ctDNA 检测的临床应用提供建议。