Flavobacterium psychrophilum, the causative agent of bacterial coldwater disease, causes substantial economic losses in salmonid farms and hatcheries. Some multilocus sequence types (ST) of F. psychrophilum are more likely to be associated with fish farms and hatcheries, but it is unclear if these patterns of association represent genetic lineages that are more adapted to aquaculture environments. Towards elucidating the disease ecology of F. psychrophilum, the culturability of 10 distinct F. psychrophilum STs was evaluated for 13 weeks in three microcosms including sterilized well water, sterilized well water with commercial trout feed, or sterilized well water with raceway detritus. All STs remained culturable in each of the microcosms for at least 8 weeks, with bacterial concentrations often highest in the presence of raceway detritus. In addition, most (e.g., 90%) STs remained culturable for at least 13-weeks. Significant differences in log₁₀ cfus were observed among STs, both within and between microcosms, suggesting potential variability in environmental persistence capacity among specific variants. Collectively, results highlight the ability of F. psychrophilum to not only persist for weeks under nutrient-limited conditions but also thrive in the presence of organic substrates common in fish farms and hatchery-rearing units.

中文翻译：

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异源嗜冷黄杆菌遗传变异在模拟养鱼场和孵化场环境的微观世界中的持续存在

嗜冷黄杆菌是细菌性冷水病的病原体，给鲑鱼养殖场和孵化场造成巨大的经济损失。F. psychrophilum的一些多位点序列类型 (ST)更有可能与养鱼场和孵化场相关，但尚不清楚这些关联模式是否代表更适合水产养殖环境的遗传谱系。为了阐明F. psychrophilum的疾病生态学，我们对 10 种不同的F. psychrophilum ST 在三个微观世界中的可培养性进行了 13 周的评估，包括无菌井水、含有商业鳟鱼饲料的无菌井水或含有水道碎屑的无菌井水。所有 ST 在每个微观世界中都保持可培养至少 8 周，在存在水道碎屑的情况下细菌浓度通常最高。此外，大多数（例如 90%）ST 仍可培养至少 13 周。在微观世界内部和微观世界之间，ST 之间观察到log ₁₀ cfu 的显着差异，表明特定变体之间的环境持久能力存在潜在差异。总的来说，结果强调了嗜冷菌不仅能够在营养有限的条件下存活数周，而且能够在养鱼场和孵化场饲养单位常见的有机基质存在下茁壮成长。