Nature Photonics ( IF 35.0 ) Pub Date : 2024-01-05 , DOI: 10.1038/s41566-023-01363-1 David Pile
Now, Matthew Peters and colleagues at the University of Victoria, Canada, and Nottingham Trent University, United Kingdom, have demonstrated real-time image tracking (see image) of unmodified proteins down to 14 kDa (ACS Nanosci. Au. https://doi.org/10.1021/acsnanoscienceau.3c00045 (2023)). This was achieved by interference imaging enhanced by surface plasmons, using just an optical microscope, an 850-nm laser, a gold metal film, and without the aid of fluorescence. The team also trapped diffusing proteins with a nanoaperture on the metal film and rapidly characterized the protein via power spectral density and noise amplitude analysis.
中文翻译:
无荧光追踪微小蛋白质
现在,加拿大维多利亚大学和英国诺丁汉特伦特大学的 Matthew Peters 及其同事展示了对低至 14 kDa 的未修饰蛋白质的实时图像跟踪(参见图片)(ACS Nanosci.Au。https :// doi.org/10.1021/acsnanoscienceau.3c00045 (2023))。这是通过表面等离激元增强的干涉成像实现的,仅使用光学显微镜、850 nm 激光、金金属薄膜,并且没有荧光的帮助。该团队还在金属薄膜上捕获了具有纳米孔径的扩散蛋白质,并通过功率谱密度和噪声幅度分析快速表征了蛋白质。